miR-200a在成釉细胞瘤中的表达及意义

›› 2012, Vol. 3 ›› Issue (1): 10-12.

miR-200a在成釉细胞瘤中的表达及意义

郭艳,魏振辉,钟鸣

中国医科大学口腔医学院

收稿日期:2011-10-17 修回日期:2011-12-22 出版日期:2012-03-25 发布日期:2012-03-31
通讯作者: 钟鸣 E-mail:zhongming_oral@yahoo.com.cn
基金资助:
中国医科大学口腔医学院青年科研启动基金项目

Expression and role of miR-200a in ameloblastoma

Received:2011-10-17 Revised:2011-12-22 Online:2012-03-25 Published:2012-03-31

摘要/Abstract

摘要： [摘要] 目的：研究miR-200a的表达与人成釉细胞瘤之间的相关性。方法：应用RNAhybrid和miRanda软件预测β-catenin基因可能的靶向miRNA；选择30例人成釉细胞瘤标本和5例正常牙胚标本，应用实时荧光定量PCR方法检测标本中miR-200a的表达情况。结果：RNAhybrid和miRanda软件分别在β-catenin mRNA第68～96碱基和第69～94碱基位置预测到一个miR-200a结合位点。与正常牙胚组织标本相比，miR-200a在人成釉细胞瘤组织标本中的表达存在明显的下调，差异显著（P < 0.05）。结论：miR-200a的表达改变与人成釉细胞瘤相关，miR-200a可能为β-catenin的靶基因之一。

Abstract: [Abstract] Objective：To study the relationship between miR-200a expression and human ameloblastoma. Methods：RNAhybrid and miRanda software were applied to predict the β-catenin targeting miRNAs. 30 cases of human ameloblastoma and 5 cases of normal tooth germ tissues were selected to detect the expression of miR-200a by real-time PCR technology. Results：MiRNA targeting prediction showed that both the 68th to 96th base pairs and the 69th to 94th base pairs in β-catenin mRNA contained a miR-200a binding site. Compared with normal tooth germ tissues, the expression of miR-200a showed an obvious down-regulation in human ameloblastoma tissues, which difference was statistically significant (P < 0.05). Conclusions：There is a relationship between the expression of miR-200a with human ameloblastoma, and miR-200a might be one of the targeting gene of β-catenin.

中图分类号:

R739.8

郭艳魏振辉钟鸣. miR-200a在成釉细胞瘤中的表达及意义[J]. , 2012, 3(1): 10-12.