大鼠正畸牙移动中Osterix的表达

›› 2012, Vol. 3 ›› Issue (1): 30-33.

大鼠正畸牙移动中Osterix的表达

朱林，李琥，夏文倩，刘翔，陈文静，邵胜，林汤毅

南京医科大学口腔医学研究所

收稿日期:2012-01-11 修回日期:2012-02-14 出版日期:2012-03-25 发布日期:2012-03-31
通讯作者: 陈文静 E-mail:chenwenjing_orth@163.com

Expression of Osterix in periodontal ligament during orthodontic tooth movement

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Received:2012-01-11 Revised:2012-02-14 Online:2012-03-25 Published:2012-03-31

摘要/Abstract

摘要： 目的：通过观察大鼠正畸牙移动过程中牙周膜内转录因子Osterix (Osx)的表达，初步探讨Osx与正畸矫治过程中牙周组织改建的关系。方法：将54只雄性SD大鼠随机分为9组，每组6只，即正畸加力0、3、6、12、24 h和3、5、7、14 d组，以右侧上颌第一磨牙不加力为自身对照组，左侧上颌第一磨牙为实验组，使用自制的加力装置移动磨牙并制备组织标本。 SABC免疫组织化学法检测实验性大鼠正畸牙齿移动过程中牙周组织Osx的表达。结果：对照组大鼠牙周组织中Osx低表达，实验组于加力5 d时Osx表达水平达到最高，且张力区整体上比压力区阳性染色深。结论：正畸力作用下Osx参于牙周组织的改建，是正畸成骨的调控途径之一。

Abstract: Objective: To explore the relationship between Osterix (Osx) and the remodeling of periodontal tissue via observing the expression of Osx in rats periodontal ligaments during the orthodontic tooth movement．Methods: 54 male SD rats were randomly divided into 9 groups, according to tooth movement time points of 0,3,6,12,24 h and 3,5,7,14 d. The orthodontic appliance was placed between the upper left first molar and the upper incisors of each rat, while the upper right first molar as the control group. Osx expression in the tissue of each rat was determined by the immunohistochemical SABC method. Results: In the control group, Osx expressed at a low level in the rat periodontal ligament.While in the experimental group, Osx expression reached the maximum after 5 d, furthermore, the Osx immunoreactivity was higher at the tension side than that at the compression side．Conclusions: Osx played an important role in alveolar bone remodeling during the orthodontic tooth movement．

朱林, 李琥, 夏文倩, 刘翔, 陈文静, 邵胜, 林汤毅. 大鼠正畸牙移动中Osterix的表达[J]. , 2012, 3(1): 30-33.

ZHU Lin, LI Hu, XIA Wen-Qian, LIU Xiang, CHEN Wen-Jing, SHAO Sheng, LIN Tang-Yi. Expression of Osterix in periodontal ligament during orthodontic tooth movement[J]. , 2012, 3(1): 30-33.