Abstract: Objective：To prepare chitosan thermosensitive hydrogel for slow release both baicalin and bovine serum albumin (BSA), and to observe the release properties vitro. Methods：The baicalin-gelatin microspheres (GMS) were prepared by chemical emulsion polycondensation. The chitosan thermosensitive hydrogel system was prepared by using different proportions of chitosan solution and β-GP, observed gelation at 37℃, and selected the best ratio. On this basis, the different concentrations of baicalin-GMS and BSA were incorporated into the chitosan thermosensitive hydrogel system. Results: Baicalin-GMS were prepared successfully, and the rate of drug loading and encapsulation efficiency was respectively 5.62% and 72.05%. The ideal state of the gel was formed by 1.8% chitosan solution and 9% after 10 min. When two drugs were incorporated into chitosan thermosensitive hydrgel, There was no apparent change in the time of phase transition of gel solution. The low concentration group cumulative release was 63.79% afer 30 days and the two higher concentrations were released 74.86%, 77.63%. Conclusions：Chitosan thermosensitive hydrogel system can simultaneously loaded baicalin-GMS and BSA, melt at room temperature. It can be transformed into semi-solid gel at 37℃ after 10 min, and drug release up to more than 30 d in vitro.