Abstract: Objective：To determinate the production of farnesol which is secreted by Candida albicans biofilms in different medium. Methods：Candida albicans SC5314 were vaccinated in RPMI1640, YPD and RPMI1640+10%FCS medium to form biofilms. At 1.5, 3, 6, 12, 24 and 48 h, farnesol was detected by gas chromatography-mass spectrometry. Results：In RPMI1640 and YPD medium, farnesol gradually increased with the biofilms development. Between the late stage of biofilms development and the mature stage, the production achieved to a high peak. In RPMI1640+10%FCS medium, farnesol gradually increased in the period of 48 hours. At 12 h, the production of farnesol in YPD was more than the others（P＜0.05）. At 48 h, the production of farnesol in RPMI1640+10%FCS was the highest（P＜0.05）. Conclusions：The medium play an important role in the production of farnesol by Candida albicans biofilms.