牙源性角化囊肿中survivin的表达及其与caspase-3和p53的关系

›› 2012, Vol. 3 ›› Issue (3): 132-134.

牙源性角化囊肿中survivin的表达及其与caspase-3和p53的关系

胡永¹,朱丽芳²,吴煜农³,刘来奎⁴

1. 苏州九龙医院
2. 苏州大学附属第一医院口腔科
3. 南京医科大学附属口腔医院
4. 南京医科大学附属口腔医院基础教研室

收稿日期:2012-07-25 修回日期:2012-08-15 出版日期:2012-09-25 发布日期:2012-10-08
通讯作者: 胡永 E-mail:huyong8044@yahoo.cn
基金资助:
苏州工业园区科技发展资金

Expression of survivin and its relationship with the expression of caspase-3 and p53 proteins in odontogenic keratocysts

Received:2012-07-25 Revised:2012-08-15 Online:2012-09-25 Published:2012-10-08
Contact: Yong HU E-mail:huyong8044@yahoo.cn

摘要/Abstract

摘要： 目的：初步探讨凋亡相关蛋白Survivin、Caspase-3和p53在牙源性角化囊肿（odontogenic keratocyst，OKC）中的表达及关系。方法：TUNEL法检测OKC中凋亡细胞的分布；免疫组织化学方法检测40例OKC（原发20例，复发20例）中Survivin、Caspase-3和p53蛋白的表达。结果：OKC中凋亡细胞见于衬里上皮表层细胞；Survivin蛋白阳性染色见于26例（65％）OKC上皮基底层及基底上层细胞中，Caspase-3蛋白阳性染色见于18例（45％）OKC上皮表层细胞中，p53蛋白在OKC中不表达。结论：凋亡相关蛋白Survivin和Caspase-3在OKC形成和发展中发挥一定作用。

Abstract: Objective：To investigate the expression of apoptosis-associated protein Survivin in human odontogenic keratocyst (OKC) and its relationship with the expression of Caspase-3 and p53. Methods：TUNEL was used to detect the distribution of apoptotic cells in OKC. Immunohistochemical assay was used to detect the expression of Survivin, Caspase-3, p53 proteins in 40 cases of OKC ( including 20 cases of primary OKC, 20 cases of recurrent OKC). Results：Apoptotic cells were mainly seen in cells of surface layer of OKC epithelial lining . Survivin positive staining was mainly in basal and suprabasal layer cells of 26（65%）cases, Caspase-3 positive staining were mainly in surface epithelial cells of 18（45%）cases. The expression of p53 was negative in OKC of all cases. Conclusions：Apoptosis-associated protein Survivin and Caspase-3 play an important role in generation and development of OKC.

中图分类号:

R739.8

胡永朱丽芳吴煜农刘来奎. 牙源性角化囊肿中survivin的表达及其与caspase-3和p53的关系[J]. , 2012, 3(3): 132-134.

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