氯化锂对毛囊神经嵴细胞增殖影响的研究

›› 2013, Vol. 4 ›› Issue (1): 31-34.

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氯化锂对毛囊神经嵴细胞增殖影响的研究

单腾飞¹,周晨²,傅瑜¹,江宏兵¹

1. 南京医科大口腔医学研究所；南京医科大学附属口腔医院口腔颌面外科
2. 无锡市第四人民医院口腔科

收稿日期:2012-08-30 修回日期:2012-11-07 出版日期:2013-03-25 发布日期:2013-03-30
通讯作者: 江宏兵 E-mail:jhbtooth@yahoo.com.cn
基金资助:
江苏省医学重点学科

LiCl effects on proliferation of hair follicles nervous crest cells

Received:2012-08-30 Revised:2012-11-07 Online:2013-03-25 Published:2013-03-30

摘要/Abstract

摘要： 目的: 研究氯化锂（LiCl）内源性激活小鼠触须垫区毛囊神经嵴细胞（Neure Crest Cells, NCCs）Wnt/β-catenin信号通路对NCCs增殖的影响。方法: 利用细胞免疫荧光及Western blot检测20mM LiCl刺激NCCs 24h后β-catenin的表达情况及细胞周期蛋白CyclinD1和CylinE1的表达情况。流式细胞技术检测不同浓度的LiCl作用不同时间后对NCCs增殖的影响。结果: LiCl刺激NCCs后，β-catenin在细胞内含量升高同时在核内积聚，CyclinD1和CylinE1表达量升高。20mM的LiCl对NCCs作用24h，NCCs表现出最强的增殖活性。结论: LiCl激活Wnt/β-catenin信号通路从而促进NCCs增殖。

Abstract: Objective To study the effects of LiCl endogenous activation of Wnt/β-catenin signaling pathways of neure crest cells (NCCs) in mice tentacles mat area on NCCs proliferation. Methods Using immunofluorescence and western blot to observe the expression of β-catenin by 20mM/L LiCl for 24h. Using western blot to evaluate the expression of CyclinD1 and CyclinE1 in NCCs by LiCl for 24h. Using FCM to investigate the effects of different concentration and different time of LiCl on cell proliferation. Results The level of β-catenin, CyclinD1 and CyclinE1 were increased in NCCs. The strongest proliferation activity of NCCs took place after 24h by 20mM LiCl. Conclusions LiCl can activate Wnt/β-catenin signaling pathway and promote NCCs proliferation.

中图分类号:

R329028

单腾飞周晨傅瑜江宏兵. 氯化锂对毛囊神经嵴细胞增殖影响的研究[J]. , 2013, 4(1): 31-34.

参考文献

[1]Chai Y, Jiang X, Ito Y, et al.Fate of the mammalian cranial neural crest during tooth and mandibular morphogenesis[J].Development,2000,127(8):1671-1679.
[2]Sieber-Blum M.Epidermal neural crest stem cells and their use in mouse models of spinal cord injury[J].Brain Res Bull,2010,83(5):189-193.
[3]Chung IH, Yamaza T, Zhao H, et al.Stem cell property of postmigratory cranial neural crest cells and their utility in alveolar bone regeneration and tooth development[J].Stem Cells,2009,27(4):866-877.
[4]Yu H, Fang D, Kumar SM, et al.Isolation of a novel population of multipotent adult stem cells from human hair follicles[J].Am J Pathol,2006,168(6):1879-1888.
[5]Clevers H, Nusse R.Wnt/β-catenin signaling and disease[J].Cell,2012,149(6):1192-1205.
[6]Wodarz A, Nusse R.Mechanisms of Wnt signaling in development[J].Annu Rev Cell Dev Biol,1998,14:59-88.
[7]Lluis F, Pedone E, Pepe S, et al.Periodic activation of Wnt/beta-catenin signaling enhances somatic cell reprogramming mediated by cell fusion[J].Cell Stem Cell,2008,3(5):493-507.
[8]Yang Y,Yang J,Liu R,et al.Accumulation of β-catenin by lithium chloride in porcine myoblast cultures accelerates cell differentiation[J].Mol Biol Rep,2011,38(3):2043-2049.
[9]Silva AK, Yi H, Hayes SH, et al.Lithium chloride regulates the proliferation of stem-like cells in retinoblastoma cell lines: a potential role for the canonical Wnt signaling pathway[J].Mol Vis,2010,16:36-45.
[10]Sieber-Blum M, Grim M, Hu YF, et al.Pluripotent neural crest stem cells in the adult hair follicle[J].Dev Dyn,2004,231(2):258-269.
[11]杨蓉, 严飞, 袁华, 等.毛囊bulge区神经嵴细胞体外分离培养及生物学特点[J].口腔医学,2009,29(5):239-241.
[12]严飞, 李君, 周晨, 等.毛囊区神经嵴干细胞成骨分化诱导的实验研究[J].口腔生物医学,2010,1(3):124-128.
[13]Clevers H.Wnt/beta-catenin signaling in development and disease[J].Cell,2006,127(3):469-480.
[14]Schou M.Lithium treatment at 52[J].J Affect Disord,2001,67(1-3):21-32.
[15]Lange C, Huttner WB, Calegari F.Cdk4/cyclinD1 overexpression in neural stem cells shortens G1, delays neurogenesis, and promotes the generation and expansion of basal progenitors[J].Cell Stem Cell,2009,5(3):320-331.
[16]Nevzorova YA, Bangen JM, Hu W, et al.Cyclin E1 controls proliferation of hepatic stellate cells and is essential for liver fibrogenesis in mice[J].Hepatology,2012,56(3):1140-1149.

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