正畸力作用下大鼠牙周组织中骨桥蛋白的表达

›› 2013, Vol. 4 ›› Issue (2): 79-82.

正畸力作用下大鼠牙周组织中骨桥蛋白的表达

韩旻轩¹,李璐²,王震东³,王珊³,吴拓江³,李琥³,林汤毅³,邵胜³,王林¹,徐艳¹

1. 南京医科大学附属口腔医院正畸科
2. 南京医科大学附属口腔医院牙周科
3. 南京医科大学附属口腔医院

收稿日期:2013-03-07 修回日期:2013-04-23 出版日期:2013-06-25 发布日期:2013-06-26
通讯作者: 王林 E-mail:nydwlktz@gmail.com
基金资助:
江苏省普通高校研究生科研创新计划项目;国家自然科学基金项目;国家自然科学基金项目;国家自然科学基金项目;江苏省科技厅项目;教育部博士点基金项目;江苏省高校优势学科建设工程项目

Expression of osteopontin in periodontal ligament during orthodontic tooth movement

Received:2013-03-07 Revised:2013-04-23 Online:2013-06-25 Published:2013-06-26

摘要/Abstract

摘要： 目的：观察SD大鼠正畸牙齿移动过程中牙周组织内骨桥蛋白(osteopontin,OPN)的表达，探讨OPN在正畸牙齿移动过程及成骨方面的作用和机制。方法：选用6~8周龄雄性SD大鼠40只，以左侧上颌第一磨牙为实验组，右侧上颌第一磨牙不加力为对照组，通过自制的加力装置牵引移动大鼠的磨牙，分别在加力后8h、24h、3d、7d处死实验动物，即刻取上颌骨制备组织标本，通过免疫组织化学方法检测SD大鼠牙周组织中OPN的表达。结果：实验组大鼠牙周膜中张力侧OPN表达明显高于对照组，3d、7d组差异有统计学意义。结论：在正畸治疗牙齿移动过程中，OPN参与牙周组织的改建并与新骨的形成相关。

Abstract: [Abstract] Objective: The purpose of this research is to study the expression changes of OPN in periodontal ligament of rats during orthodontic tooth movement. Methods: 40 SD rats were randomly grouped according to tooth movement days, such as 8h,24h,3d,7d. The orthodontic appliance was placed between the upper incisors and the upper left first molar of each rat, while those whose upper right first molar has no orthodontic appliance as the control group．The expressions of OPN were performed by immunohistochemistry. Results: Significant expressions of OPN were found in periodontal fibroblasts of the tension side after mechanical stress, while the control group OPN was expressed at a low level in the periodontal ligament. Conclusions: OPN plays a key role in alveolar bone remodeling during orthodontic tooth movement.

中图分类号:

R329.28

韩旻轩李璐王震东王珊吴拓江李琥林汤毅邵胜王林徐艳. 正畸力作用下大鼠牙周组织中骨桥蛋白的表达[J]. , 2013, 4(2): 79-82.

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