let-7基因多态与中国汉族人群头颈癌易感性的关联研究

›› 2014, Vol. 5 ›› Issue (2): 57-60.

• 论著 • 下一篇

let-7基因多态与中国汉族人群头颈癌易感性的关联研究

朱龙彪¹,袁华²,张宇¹,苗利民¹,陈宁²

1. 南京医科附属口腔医院
2. 南京医科大学口腔医学院

收稿日期:2014-02-14 修回日期:2014-05-19 出版日期:2014-06-25 发布日期:2014-06-23
通讯作者: 陈宁 E-mail:njcn@njmu.edu.cn
基金资助:
国家自然科学基金;国家重点基础研究发展计划(973 计划);江苏省自然科学基金;江苏高校优势学科建设工程资助项目;2013年高等学校博士学科点专项科研基金

Investigation of correlation between genetic polymorphism of let-7 and genetic susceptibility to head and neck cancer risk in Han Chinese

Received:2014-02-14 Revised:2014-05-19 Online:2014-06-25 Published:2014-06-23

摘要/Abstract

摘要： 目的：探讨let-7基因多态与中国汉族人群头颈癌易感性的关联。方法：采用病例－对照研究设计，以经确诊的503例头颈部癌患者作为病例组，选取900例健康人群作为对照组。对病例－对照进行流行病学调查，内容包括：一般人口学特征、疾病史、肿瘤家族史、吸烟、饮酒情况，并进行体格检查。收集研究对象血液标本5 mL，提取基因组DNA。以let-7 rs10877887和rs13293512为研究位点，应用TaqMan探针方法进行多态性检测，并用logistic回归计算比值比（odds ratio，OR）及其95%可信区间（confidence interval，CI），比较不同基因型与头颈癌患病风险的关系。结果：rs10877887位点3种基因型TT、CT及CC在病例组分布频率分别为45.7%（227/503）、42.9%（213/503）及11.4%（57/503）；在对照组中分别为40.8%（361/900）、47.7%（422/900）及11.5%（101/900）。rsl3293512位点3种基因型TT、CT及CC在病例组分布频率分别为31.9%（157/503）、52.3%（257/503）及15.8%（78/503），在对照组中分别为30.2%（270/900）、49.2%（439/900）及20.6%（194/900）。多因素logistic回归分析显示，携带rs10877887位点至少1个突变等位基因C的个体与携带TT基因型的个体相比，头颈部肿瘤患病风险差异无统计学意义（CC+CT/TT调整OR=0.82，95% CI：0.90~1.23，P=0.087）；与携带TT+CT基因型个体相比较，携带rs13293512位点2个突变等位基因C的个体头颈部肿瘤患病风险显著降低（CC/TT+CT调整OR=0.73，95% CI：0.55~0.98，P=0.039）。结论：let-7基因多态rs13293512位点多态位点改变可影响中国汉族人群的罹患头颈癌的风险。

Abstract: Objective: To discuss the association between single nucleotide polymorphisms in let-7 and genetic susceptibility to head and neck cancer (HNC) in Han Chinese. Methods: In this case-control study, 503 newly and histologically confirmed HNC patients were consecutively recruited as cases and 900 cancer-free controls were randomly selected as controls. Designed questionnaires were used to acquire patients’ information on demographic data and age, sex, smoking, and alcohol consumption. Approximate 5 ml of venous blood sample was collected from each subject for DNA extraction and genotyping. We used TaqMan allelic discrimination assay to genotype two polymorphisms of rs10877887 and rs13293512 in let-7. The associations of SNPs with HNC risks were estimated by computing the crude and adjusted odds ratios (ORs) and their 95% confidence intervals (CIs) using logistic regression analyses. Results: The frequencies of genotype TT, CT and CC of rs10877887 were 45.7% (227/503), 42.9% (213/503) and 11.4% (57/503) in case group; while separately 40.8% (361/900), 47.7% (422/900) and 11.5% (101/900) in control group. The frequencies of genotype TT, CT and CC of rs13293512 were 31.9% (157/503), 52.3% (257/503) and 15.8% (78/503); while separately 30.2% (270/900), 49.2% (439/900) and 20.6% (194/900) in control group. Logistic regression analyses showed that there was no statistic significe in the relationship between rs10877887 and susceptibility to HNC (CC+CT/TT：adjusted OR=0.82, 95% CI: 0.90-1.23, P=0.087), while rs13293512 CC genotype significantly decreased susceptibility to HNC (CC/TT+CT：adjusted OR=0.73, 95% CI：0.55-0.98, P=0.039). Conclusions: The polymorphism of rs13293512 in let-7 may be a biomarker of susceptibility to HNC in Han Chinese.

朱龙彪袁华张宇苗利民陈宁. let-7基因多态与中国汉族人群头颈癌易感性的关联研究[J]. , 2014, 5(2): 57-60.

参考文献

[1]Jemal A, Tiwari RC, Murray T, et al.Cancer statistics,2004[J].CA Cancer J Clin,2004,54(1):8-29.
[2]Hecht SS.Tobacco carcinogens, their biomarkers and tobacco-induced cancer[J].Nat Rev Cancer,2003,3(10):733-744.
[3]D'Souza G, Kreimer AR, Viscidi R, et al.Case-control study of human papillomavirus and oropharyngeal cancer[J].N Engl J Med,2007,356(19):1944-1956.
[4]Ambros V.The functions of animal microRNAs[J].Nature,2004,431(7006):350-355.
[5]Xu Y, Liu L, Liu J, et al.A potentially functional polymorphism in the promoter region of miR-34b/c is associated with an increased risk for primary hepatocellular carcinoma[J].Int J Cancer,2011,128(2):412-417.
[6]Hu Z, Chen J, Tian T, et al.Genetic variants of miRNA sequences and non-small cell lung cancer survival[J].J Clin Invest,2008,118(7):2600-2608.
[7]Yu CC, Chen YW, Chiou GY,et al.MicroRNA let-7a represses chemoresistance and tumourigenicity in head and neck cancer via stem-like properties ablation[J].Oral Oncol,2011,47(3):202-210.
[8]Takamizawa J, Konishi H, Yanagisawa K, et al.Reduced expression of the let-7 microRNAs in human lung cancers in association with shortened postoperative survival[J].Cancer Res,2004,64(11):3753-3756.
[9]Wang S, Tang Y, Cui H, et al.Let-7/miR-98 regulate Fas and Fas-mediated apoptosis[J].Genes Immun,2011,12(2):149-154.
[10]Calin GA, Sevignani C, Dumitru CD, et al.Human microRNA genes are frequently located at fragile sites and genomic regions involved in cancers[J].Proc Natl Acad Sci U S A,2004,101(9):2999-3004.
[11]Mayr C, Hemann MT, Bartel DP.Disrupting the pairing between let-7 and Hmga2 enhances oncogenic transformation[J].Science,2007,315(5818):1576-1579.
[12]Yang J, Zhou F, Xu T, et al.Analysis of sequence variations in 59 microRNAs in hepatocellular carcinomas[J].Mutat Res,2008,638(1-2):205-209.
[13]Chang CJ, Hsu CC, Chang CH, et al.Let-7d functions as novel regulator of epithelial-mesenchymal transition and chemoresistant property in oral cancer[J].Oncol Rep,2011,26(4):1003-1010.

let-7基因多态与中国汉族人群头颈癌易感性的关联研究

Investigation of correlation between genetic polymorphism of let-7 and genetic susceptibility to head and neck cancer risk in Han Chinese

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 0

编辑推荐

Metrics

本文评价