两种保存液对脱位牙再植术效果影响的实验研究

›› 2014, Vol. 5 ›› Issue (2): 85-90.

两种保存液对脱位牙再植术效果影响的实验研究

朱泽群¹,陈铭清¹,刘茜¹,蔡琴¹,王珏²,周红艳³,⁴,梅予锋⁵

1. 南京医科大学口腔医学研究所
2. 江苏省口腔医院
3.
4. 江苏省儿童预防牙病科
5. 南京医科大学

收稿日期:2014-01-13 修回日期:2014-04-29 出版日期:2014-06-25 发布日期:2014-06-23
通讯作者: 梅予锋 E-mail:yfmei@njmu.edu.cn

Experimental research into the influence of two kinds of storage media on the replantation of avulsed tooth

Received:2014-01-13 Revised:2014-04-29 Online:2014-06-25 Published:2014-06-23

摘要/Abstract

摘要： 【摘要】目的比较常温下脱脂牛奶与汉克斯平衡盐溶液（Hank’s Balanced Salt Solution, HBSS）保存脱位牙对再植牙牙根愈合过程的影响。方法 18只6周龄SPF级Wistar雄性大鼠，上颌第一磨牙脱位后分别采用HBSS、脱脂牛奶或干燥（阴性对照）室温保存,30min后植回牙槽窝，分别于术后1、3、7、14、21d处死大鼠，拍摄根尖x线片进行图像分析;标本切片进行组织学观察。结果再植后各时间点牛奶组与HBSS组近中根阴影面积无统计学差异，皆小于干燥组（P<0.01）；牛奶组根尖组织炎症反应较轻，根吸收少，根周修复类型与HBSS基本相同。结论脱脂牛奶与HBSS在常温下均为良好的脱位牙体外保存液，可在普通人群中作为再植牙保存液推广使用。

Abstract: [Abstract] Objective: To evaluate the effect of skimmed milk and Hank’s Balanced Salt Solution(HBSS) as preservation medium on the root healing and root resorption of the avulsed tooth. Methods: 60 bilateral maxillary first molars was extracted from 30 6-week-old SPF Wistar male rats which were divided randomly into 5 groups (6 each group), and replanted back into the sockets after being placed in HBSS or skimmed milk or in the air ( as negative control) for 30 min right after extraction. The rats were sacrificed on day1,day 3,day7,day14 and day21 after the operation. Then the maxillae were scanned with X-ray and the shadow areas around the mesial roots of maxillary first molars were measured with IPP software. After decalcification, the specimens were made into slices and stained with HE for histological observation. Results: There was no statistically significant difference between milk group and HBSS group regarding the shadow area around mesial roots；the inflammation of root tissue in milk group came to a lighter degree with less resorption, and both the two groups had periodontal healing. Conclusions: Skimmed milk can be recommended as suitable substitute for HBSS and promoted in large-scale.

朱泽群陈铭清刘茜蔡琴王珏周红艳梅予锋. 两种保存液对脱位牙再植术效果影响的实验研究[J]. , 2014, 5(2): 85-90.

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