›› 2015, Vol. 6 ›› Issue (1): 1-5.

• 论著 •    下一篇

法尼醇对不同状态白念珠菌氟康唑药物敏感性作用的比较研究

曹雪姣1,吴亚娟2,花荣3,魏昕4   

  1. 1. 南京医科大学口腔研究所
    2. 南京医科大学口腔医学院
    3. 南京医科大学附属口腔医院牙体牙髓病科;南京医科大学口腔医学研究所
    4. 江苏省口腔医院
  • 收稿日期:2015-01-05 修回日期:2015-02-08 出版日期:2015-03-25 发布日期:2015-04-08
  • 通讯作者: 魏昕 E-mail:weixinart@163.com
  • 基金资助:
    国家自然科学基金;国家自然科学基金

Comparative study on the sensitivity of Candida albicans in different states to fluconazole treated with farnesol

  • Received:2015-01-05 Revised:2015-02-08 Online:2015-03-25 Published:2015-04-08

摘要: 目的:探讨法尼醇处理对不同生长状态白念珠菌的氟康唑药物敏感性的影响。方法:体外构建浮游状态白念珠菌和成熟生物膜状态白念珠菌;法尼醇处理后,用甲基四氮盐(XTT)法检测浮游状态和生物膜状态白念珠菌细胞活性的改变。结果:与对照组比较,法尼醇处理后的浮游状态白念珠菌对氟康唑的敏感性无明显改变(P>0.05),而生物膜状态白念珠菌对氟康唑的敏感性增高(P<0.05)。结论:法尼醇可以增加白念珠菌生物膜对药物的敏感性。

Abstract: Objective: To explore the effect of farnesol on sensitivity to fluconazole of Candida albicans (C.albicans) in different states. Methods:We used XTT reduction assay to test the inhibitory effects of farnesol on the C.albicans planktonic and biofilms in vitro. The research included four groups:farensol-treated planktonic group, farnesol-free planktonic group, farnesol-treated biofilms group and farnesol-free biofilms group. Results:The XTT analysis suggested there is no significant difference on the antifungal suscepitibility of farnesol-treated planktonic and farnesol-free planktonic when treated with fluconazole (P>0.05), but there is remarkable difference on farnesol-treated biofilm and farnesol-free biofilm when treated with flucoazole (P<0.05). Conclusions:Farnesol can increase the antifungal suscepitibility of C. albicans biofilm.