中药黄芩苷对牙髓干细胞增殖及成牙成骨分化能力的影响

›› 2015, Vol. 6 ›› Issue (1): 11-14.

中药黄芩苷对牙髓干细胞增殖及成牙成骨分化能力的影响

薛晶¹,俞艳²,王利娟³,于金华¹,张光东⁴

1. 南京医科大学
2. 南京医科大学口腔医学研究所
3. 常州市口腔医院
4. 南京医科大学口腔医学院

收稿日期:2015-01-08 修回日期:2015-03-07 出版日期:2015-03-25 发布日期:2015-04-08
通讯作者: 张光东 E-mail:egd_zhang@njmu.edu.cn
基金资助:
江苏高校优势学科建设工程资助项目

Effect of baicalin on the proliferation and odento/osteogenic differentiation of human dental pulp stem cells

1. Nanjing Medical University
2. Institute of Stomatology, School of Stomatology, Nanjing Medical University
3. Stomatological Hospital of Changzhou
4.

Received:2015-01-08 Revised:2015-03-07 Online:2015-03-25 Published:2015-04-08

摘要/Abstract

摘要： 目的：探讨中药黄芩苷对牙髓干细胞的增殖、成牙/成骨分化能力的影响。方法：酶消化法原代培养人牙髓干细胞，采用甲基噻唑基四唑（MTT）比色法检测黄芩苷对人牙髓干细胞增殖的影响，碱性磷酸酶活性检测及Western blot等方法检测黄芩苷作用于人牙髓干细胞后，其成牙/成骨分化指标，包括核心结合因子（runt-related transcription factor 2，RUNX2）、牙本质涎蛋白（dentin sialoprotein，DSP）、Osterix（OSX）、骨桥蛋白（osteopontin，OPN）、骨钙素（osteocalcin，OCN）的变化。结果：MTT检测结果显示，0~20 μmol/L黄芩苷作用于牙髓干细胞后其增殖能力与对照组相比无明显差异（P>0.05）；而碱性磷酸酶活性检测显示：0~20 μmol/L黄芩苷各浓度组细胞ALP活性比对照组明显增加（P<0.01）；Western blot结果表明：与对照组相比，20 μmol/L黄芩苷可增强牙髓干细胞RUNX2、DSP、OSX、OPN、OCN等成牙/成骨相关蛋白的表达。结论：黄芩苷对牙髓干细胞的增殖无明显影响，但可促进其成牙/成骨分化能力。

Abstract: Objective：To investigate the effect of baicalin on the proliferation and odento/osteogenic differentiation of huaman dental pulp stem cells (hDPSCs) in vitro. Methods：hDPSCs were isolated from dental pulp and then were cultured in alpha minimun essential medium(α-MEM) supplimented with or without baicalin. MTT colorimetric method was used to examine the proliferation ability of hDPSCs. The effect of baicalin on the odento/osteogenic differentiation was observed by alkaline phosphotase(ALP) activity and Western blot. Results： MTT assay showed that there was no significant difference between baicalin-treated and untreated control cells (P>0.05), and ALP activity assay demonstrated that 0~20μmol/L baicalin enhanced the ALP activity of DPSCs(P<0.01), and Western blot revealed that the odento/osteogenic marker (RUNX2, DSP, OSX, OPN, OCN) was significantly upregulated in baicalin-treated cells compared with the untreated cells. Conclusions： Baicalin has no effect on the proliferation of DPSCs, but enhances the capacity of odento/osteogenic differentiation.

中图分类号:

R780.2

薛晶俞艳王利娟于金华张光东. 中药黄芩苷对牙髓干细胞增殖及成牙成骨分化能力的影响[J]. , 2015, 6(1): 11-14.

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