O-糖基化在口腔鳞状细胞癌发生发展中的作用初探

›› 2015, Vol. 6 ›› Issue (4): 172-177.

O-糖基化在口腔鳞状细胞癌发生发展中的作用初探

李怡宁,陈思远,胡济安

浙江大学医学院附属口腔医院病理科

收稿日期:2015-10-29 修回日期:2015-11-24 出版日期:2015-12-25 发布日期:2015-12-30
通讯作者: 胡济安 E-mail:hja@zju.edu.cn
基金资助:
国家自然科学基金青年科学基金项目;浙江省中医药科学研究基金

Research on the relationship between O-GlcNAc and oral squamous cell carcinoma

Received:2015-10-29 Revised:2015-11-24 Online:2015-12-25 Published:2015-12-30

摘要/Abstract

摘要： 目的：通过口腔鳞癌组织和细胞学的研究，初步探讨O-糖基化和口腔鳞状细胞癌关系。方法：对30例口腔鳞状细胞癌和10例正常口腔黏膜组织，采用免疫组织化学SP法检测O位N-乙酰葡萄糖胺（O-linked N-acetylglucosamine，O-GlcNAc）、N-乙酰氨基葡萄糖转移酶（O-GlcNAc transferase，OGT）和N-乙酰氨基葡萄糖苷酶（O-GlcNAcase，OGA）的表达，用免疫荧光、Western blotting检测在糖基化增强剂（transglycosylation，TG）和糖基化抑制剂（deoxynorleucine，DON）作用下舌鳞状细胞癌TCA8113细胞的增殖。结果：O-GlcNAc和OGT在正常口腔黏膜组织和口腔鳞癌组织中存在表达显著性差异（P<0.05），并且随着肿瘤恶性程度的增高，呈现表达升高的趋势。OGA的表达和O-GlcNAc、OGT均不同，从正常到鳞癌中表达呈增高趋势，但在不同分化鳞癌组内表达差异不明显。TG可促进TCA8113的增殖和O-GlcNAc、OGT的表达，DON可抑制TCA8113的增殖和O-GlcNAc、OGT的表达。结论：O-糖基化在口腔鳞状细胞癌中表达增高，在舌鳞癌细胞系中活化，抑制剂DON可抑制肿瘤细胞增殖。

Abstract: Objective：To investigate the relationship between O-linked N-acetylglucosamine (O-GlcNAc) and oral squamous cell carcinoma. Methods：The expression of O-GlcNAc, O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA) in 30 cases of oral squamous cell carcinoma and 10 normal oral mucosa were detected using immunohistochemical staining. The proliferation of TCA8113 with transglycosylation (TG) and deoxynorleucine (DON) was examined by Immunofluence and Western blotting. Results：There was significant difference of O-GlcNAc and OGT between normal mucosa and oral squamous cell carcinoma (P<0.05). The expression of O-GlcNAc and OGT increased with higher grade of the carcinoma. The expression of OGA was inconsistent with O-GlcNAc and OGT. TG could activate the expression of O-GlcNAc and OGT, DON could inhibit the expression of O-GlcNAc and OGT. Besides, DON could inhibit the proliferation of TCA8113 cells and the expression of PCNA. Conclusions：O-GlcNAc could activate the oral squamous cell carcinoma. Inhibitor DON could depress the proliferation of TCA8113.

李怡宁陈思远胡济安. O-糖基化在口腔鳞状细胞癌发生发展中的作用初探[J]. , 2015, 6(4): 172-177.

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