转录因子GATA4对颅面骨发育与矿化的影响

›› 2017, Vol. 8 ›› Issue (1): 16-19.

转录因子GATA4对颅面骨发育与矿化的影响

周婷婷¹,郭舒瑜¹,张宇新¹,翁亚娟²,张梦男³,马俊青²

1. 南京医科大学口腔疾病研究江苏省重点实验室，南京医科大学
2. 南京医科大学口腔疾病研究江苏省重点实验室
3. 南京医科大学附属口腔医院正畸科

收稿日期:2016-11-23 修回日期:2017-02-16 出版日期:2017-03-25 发布日期:2017-04-11
通讯作者: 马俊青 E-mail:jma@njmu.edu.cn
基金资助:
国家自然科学基金资助项目;江苏省杰出青年科学基金资助项目;江苏高校优势学科建设工程资助项目

The role of transcription factor GATA4 in craniofacial bone formation and mineralization

1. Jiangsu Key Laboratory of Oral Disease, Nanjing Medical University
2. Department of Orthodontics, Affiliated Hospital of Stomatology, Nanjing Medical University

Received:2016-11-23 Revised:2017-02-16 Online:2017-03-25 Published:2017-04-11

摘要/Abstract

摘要： 目的通过条件性基因敲除小鼠模型，探讨转录因子GATA4在颅面骨发育与矿化中的作用。方法通过Cre-loxp转基因小鼠技术，将Wnt1-Cre转基因小鼠与GATA4fl/fl转基因小鼠杂交，得到神经嵴细胞特异性GATA4基因敲除小鼠（Wnt1-Cre；GATA4fl/fl）。取2周龄雄性GATA4条件敲除小鼠和野生型小鼠各5只处死后应用Micro-CT进行颅盖骨、腭骨和右侧下颌骨的骨形态及骨密度分析。结果与野生型小鼠相比，GATA4条件敲除小鼠的颅盖骨长度、宽度、鼻额角、腭部宽度、下颌骨长度及高度均明显减小，而腭中缝宽度增大（P<0.05）。下颌骨骨小梁体积分数、骨小梁数量均下降，骨小梁间隔增加（P<0.05）。结论 GATA4参与调控了颅面部骨的发育，对颅面部骨的形成和矿化具有重要调控作用。

Abstract: Objective To explore the effect of transcription factor GATA4 on bone formation and mineralization. Methods Wnt1-Cre mice were mated to GATA4fl/fl mice by cre-loxp gene conditional knockout technique, and conditional knockout GATA4 gene of neural crest cell mice (Wnt1-Cre;GATA4fl/fl) were produced. 2-week-old GATA4 conditional knockout (CKO) mice and wild type (WT) mice were sacrificed and then Micro-CT was conducted to compare the size and volume of craniofacial bones such as calvarial bone, palate bone and right mandible bone. Results Compared with WT mice, the skull bone, palate bone and mandible bone of CKO mice were smaller, and the width of midpalatal suture was increased. The mandible of CKO mice also showed decreased BV/TV and Tb.N (P<0.05) values, and increased Tb.Sp value; Tb.N was not significantly different (P>0. 05). Conclusions These results indicate an important role of GATA4 in craniofacial bone development and mineralization.

中图分类号:

R783.5

周婷婷郭舒瑜张宇新翁亚娟张梦男马俊青. 转录因子GATA4对颅面骨发育与矿化的影响[J]. , 2017, 8(1): 16-19.

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