Acvr1敲除对牙本质形成的影响研究

›› 2017, Vol. 8 ›› Issue (3): 132-135.

Acvr1敲除对牙本质形成的影响研究

张雪¹,刘麒麟²,李媛³,赵欢¹,刘苍维⁴,闫广兴⁴,胡月¹,李道伟¹,史册¹,孙宏晨¹

1. 吉林大学口腔医学院病理科
2. 吉林大学口腔医院口腔颌面外科
3. 吉林大学口腔医院
4. 吉林大学口腔医学院口腔病理科

收稿日期:2017-06-27 修回日期:2017-08-31 出版日期:2017-09-25 发布日期:2017-10-24
通讯作者: 孙宏晨 E-mail:hcsun@mail.jlu.edu.cn
基金资助:
国家重点研发专项“新型颌面软硬组织修复材料研发”;国家自然科学基金国际（地区）合作与交流项目;国家自然科学基金青年基金项目;中国博士后科学基金

The role of BMP type I receptor ACVR1 in regulating dentin formation

Received:2017-06-27 Revised:2017-08-31 Online:2017-09-25 Published:2017-10-24

摘要/Abstract

摘要： 目的：探讨牙乳头细胞中Ⅰ型骨形成蛋白受体——活化蛋白A受体1基因（ACVR1）敲除对牙本质形成的影响。方法：以同窝Osterix-Cre; Acvr1fx/-基因型小鼠为实验组，Osterix-Cre; Acvr1fx/+小鼠为对照组，利用影像学方法和HE染色初步观察Acvr1条件性基因敲除后小鼠牙本质形成及成牙本质细胞形态；免疫组织化学染色检测成牙/成骨分化相关蛋白水平；实时定量RT-PCR技术检测成牙/成骨分化相关因子的表达。结果：micro-CT显示，实验组下颌骨表现为疏松多孔样结构，磨牙牙本质变薄，前期牙本质增厚，HE染色显示成牙本质细胞排列紊乱，而切牙出现骨样牙本质；实验组磨牙成牙本质细胞分化相关基因Dspp、Nestin表达有下调趋势（无统计学差异），切牙成牙齿分化相关基因Dspp表达有下调趋势（无统计学差异），免疫组织化学染色显示实验组磨牙DSPP表达减少。结论：ACVR1通过调控成牙本质细胞分化而影响牙本质形成，在调控成牙本质细胞成牙转化过程中具有重要作用。

Abstract: Objective：To explore the effect of knockout of Acvr1（activin A receptor type 1） on the formation of dentin. Methods： Acvr1 conditional knockout mice model was constructed using Cre-loxP system. The genotype Osterix-Cre; Acvr1fx/- was chosen as the experiment group and the genotype Osterix-Cre; Acvr1fx/+ was chosen as the control group in littermate. We observed the dentin formation and odontoblast morphology by imageology and HE staining. And we checked the expression of the odontoblast/osteoblast markers by immunohistochemical staining. Finally the expression of odontoblast/osteoblast genes was detected by qRT-PCR. Results：The mandibles of the experiment group showed more pores, the dentin of mandibular first molar was thinner, pre-dentin was thicker, and odontoblast looked irregular. The genes important for odontoblast differentiation such as Dspp, Nestin were downregulated. However, the incisor showed osteodentin, and the Dspp was down-regulated. IHC showed down-regulated DSPP in cKO molar. Conclusions：ACVR1 affected the formation of dentin by up-regulating odontoblast differentiation, and also had important role in odontoblast transition.

张雪刘麒麟李媛赵欢刘苍维闫广兴胡月李道伟史册孙宏晨. Acvr1敲除对牙本质形成的影响研究[J]. , 2017, 8(3): 132-135.

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