持续激活β-catenin对拔牙窝初期愈合的影响

口腔生物医学 ›› 2024, Vol. 15 ›› Issue (3): 140-146.

持续激活β-catenin对拔牙窝初期愈合的影响

陈艳丽¹,²,张旗³,⁴

1. Department of Endodontics, School and Hospital of Stomatology, Tongji University
2. 同济大学口腔医学院
3. 同济大学口腔医学院同济大学附属口腔医院牙体牙髓科，上海牙组织修复与再生工程技术研究中心
4. 同济大学口腔医学院·同济大学附属口腔医院牙体牙髓科

收稿日期:2024-02-29 修回日期:2024-03-22 出版日期:2024-06-25 发布日期:2024-07-04
通讯作者: 张旗 E-mail:qizhang@tongji.edu.cn
基金资助:
牙髓基质细胞线粒体转移在消退素E1介导的牙髓炎症消退及损伤修复中的作用和机制研究;牙髓感觉神经通过CGRP/Ramp1轴调控DPSCs定向迁移促牙髓损伤修复及机制研究

Effect of constitutive activation of β-catenin on initial healing of extraction sockets

1. Engineering Research Center of Tooth Restoration and Regeneration, Shanghai
2. School of Dentistry Tongji University
3.
4. Department of Endodontics, Stomatological Hospital and Dental School of Tongji University, Shanghai Engineering Research Center of Tooth Restoration and Regeneration

Received:2024-02-29 Revised:2024-03-22 Online:2024-06-25 Published:2024-07-04
Supported by:
The role and mechanism of mitochondrial transfer of dental pulp stromal cells in Resolvin E1-mediated resolution of pulp inflammation and repair of pulp injury;The mechanism of pulp sensory nerve regulating the directional migration of DPSCs through CGRP/Ramp1 axis to promote dental pulp injury repair

摘要/Abstract

摘要： [摘要]目的：探究持续激活β-catenin对拔牙后牙槽骨初期骨改建的影响。方法：构建Catnblox(ex3)/+小鼠（对照组）和DMP1-9.6kb-cre+/-;Catnblox(ex3)/+小鼠（实验组）。分别拔除两组小鼠右上颌第一磨牙，1周后收样。组织学染色观察拔牙窝愈合情况。结果：与对照组相比，实验组拔牙窝内TRAP染色显示破骨细胞数量减少（P<0.001），Masson染色显示胶原矿化减少（P<0.01）；免疫组化显示成骨相关分子OCN、DMP1、OPG特异性染色减少（P<0.01）。结论：DMP1-9.6kb-cre中持续激活β-catenin延缓拔牙窝的初期愈合速率。 [关键词] 拔牙窝愈合；牙槽骨改建；β-catenin；Wnt/β-catenin信号通路；

关键词: [关键词] 拔牙窝愈合, 牙槽骨改建, β-catenin, Wnt/β-catenin信号通路

Abstract: [Abstract] Objective:To investigate the effects of constitutive activation of β-catenin on the initial alveolar bone remodeling after tooth extraction. Methods: Catnblox(ex3)/+mice (control group) and DMP1-9.6kb-cre+/-;Catnblox(ex3)/+mice (experimental group) were constructed. The right maxillary first molar was extracted and were collected 1 week later. Histological stainings were performed to observe the healing of the extraction sockets. Results: Compared with the control group, TRAP staining of the experimental group showed a decrease in the number of osteoclasts (P<0.001), and Masson staining showed decreased collagen mineralization (P<0.01). Immunohistochemistry showed decreased specific staining for osteogenesis-related molecules OCN, DMP1, and OPG (P<0.01). Conclusion: Constitutive activation of β-catenin in DMP1-9.6kb-cre delays the initial healing rate of extraction sockets. [Key words] Extraction socket healing; Alveolar bone remodeling; β-catenin; Wnt/β-catenin signaling pathway;

Key words: [Key words] Extraction socket healing, Alveolar bone remodeling, β-catenin, Wnt/β-catenin signaling pathway

中图分类号:

R782.11

陈艳丽张旗. 持续激活β-catenin对拔牙窝初期愈合的影响[J]. 口腔生物医学, 2024, 15(3): 140-146.

参考文献 24

[1]	Chen J, He Y, Keilig L, et al. Numerical investigations of bone remodelling around the mouse mandibular molar primordia[J]. Ann Anat,2019,222;146-152.
[2]	Sodek J, Mckee M D. Molecular and cellular biology of alveolar bone[J].Periodontol 2000,2000,24;99-126.
[3]	Yin X, Li J, Salmon B, et al. Wnt Signaling and Its Contribution to Craniofacial Tissue Homeostasis[J]. J Dent Res,2015,94(11);1487-1494.
[4]	Duan P, Bonewald L F. The role of the wnt/beta-catenin signaling pathway in formation and maintenance of bone and teeth[J]. Int J Biochem Cell Biol,2016,77(Pt A);23-29.
[5]	Liu J, Xiao Q, Xiao J, et al. Wnt/beta-catenin signalling: function, biological mechanisms, and therapeutic opportunities[J]. Signal Transduct Target Ther,2022,7(1);3.
[6]	Chen J, Long F. beta-catenin promotes bone formation and suppresses bone resorption in postnatal growing mice[J]. J Bone Miner Res,2013,28(5);1160-1169.
[7]	Huang P, Yan R, Zhang X, et al. Activating Wnt/beta-catenin signaling pathway for disease therapy: Challenges and opportunities[J]. Pharmacol Ther,2019,196;79-90.
[8]	Chen S, Feng J, Bao Q, et al. Adverse effects of osteocytic constitutive activation of β-catenin on bone strength and bone growth[J].J Bone MinerRes,2015,30(7):1184-1194.
[9]	Sui X, Deng S, Liu M, et al. Constitutive activation of β-catenin in differentiated osteoclasts induces bone loss in mice[J].Cell Physiol Biochem,2018,48(5):2091-2102．
[10]	Kahla R B, Barkaoui A. Chapter2-Bone remodeling biology[M] Bone Remodeling Process, Kahla R B, Barkaoui A. Academic Press,2021.49-87.
[11]	Hathaway-Schrader J D, Novince C M. Maintaining homeostatic control of periodontal bone tissue[J]. Periodontol 2000,2021,86(1);157-187.
[12]	Zeng L, He H, Sun M, et al. Runx2 and Nell-1 in dental follicle progenitor cells regulate bone remodeling and tooth eruption[J]. Stem Cell Res Ther,2022,13(1);486.
[13]	Araujo M G, Silva C O, Misawa M, et al. Alveolar socket healing: what can we learn?[J]. Periodontol 2000,2015,68(1);122-134.
[14]	Kim J M, Lin C, Stavre Z, et al. Osteoblast-Osteoclast Communication and Bone Homeostasis[J]. Cells,2020,9(9).
[15]	Marini F, Giusti F, Palmini G, et al. Role of Wnt signaling and sclerostin in bone and as therapeutic targets in skeletal disorders[J]. Osteoporos Int,2023,34(2);213-238.
[16]	Kramer I, Halleux C, Keller H, et al. Osteocyte Wnt/beta-catenin signaling is required for normal bone homeostasis[J]. Mol Cell Biol,2010,30(12);3071-3085.
[17]	Maeda K, Kobayashi Y, Koide M, et al. The Regulation of Bone Metabolism and Disorders by Wnt Signaling[J]. Int J Mol Sci,2019,20(22).
[18]	Harada N, Tamai Y, Ishikawa T, et al. Intestinal polyposis in mice with a dominant stable mutation of the beta-catenin gene[J]. EMBO J,1999,18(21);5931-5942.
[19]	梁博龙,张武阳,薛洋等.C57BL/6小鼠上颌第一磨牙拔牙创愈合过程观察分析[J].中国实用口腔科杂志,2019,12(04):232-236.
[20]	Gwak J, Hwang S G, Park H S, et al. Small molecule-based disruption of the Axin/beta-catenin protein complex regulates mesenchymal stem cell differentiation[J]. Cell Res,2012,22(1);237-247.
[21]	Eijken M, Meijer I M, Westbroek I, et al. Wnt signaling acts and is regulated in a human osteoblast differentiation dependent manner[J]. J Cell Biochem,2008,104(2);568-579.
[22]	Price M A. CKI, there's more than one: casein kinase I family members in Wnt and Hedgehog signaling[J]. Genes Dev,2006,20(4);399-410.
[23]	Udeabor S E, Heselich A, Al-Maawi S, et al. Current Knowledge on the Healing of the Extraction Socket: A Narrative Review[J]. Bioengineering (Basel),2023,10(10).
[24]	Couso-Queiruga E, Stuhr S, Tattan M, et al. Post-extraction dimensional changes: A systematic review and meta-analysis[J]. J Clin Periodontol,2021,48(1);126-144.