MDP-钙盐对牙髓干细胞增殖和成牙本质分化的影响

口腔生物医学 ›› 2024, Vol. 15 ›› Issue (5): 252-256.

MDP-钙盐对牙髓干细胞增殖和成牙本质分化的影响

马语笛¹,谢海峰²

1. 南京医科大学附属口腔医院
2. 南京医科大学

收稿日期:2024-04-07 修回日期:2024-07-22 出版日期:2024-10-25 发布日期:2024-11-04
通讯作者: 谢海峰 E-mail:xhf-1980@126.com
基金资助:
国家自然科学基金;江苏省科教能力提升工程———江苏省研究型医院建设单位;江苏省医学创新中心

Effects of MDP-calcium salts on the proliferation and odontogenic differentiation of dental pulp stem cells

Yu-Di MA¹,

Received:2024-04-07 Revised:2024-07-22 Online:2024-10-25 Published:2024-11-04

摘要/Abstract

摘要： 目的：探索甲基丙烯酰氧基癸基磷酸二氢酯（MDP）-钙盐对牙髓干细胞（DPSCs）成牙本质分化的影响，以评估MDP-钙盐对第三期牙本质形成及牙髓保护的作用。方法：使用不同比例的10-MDP与CaCl2合成三种MDP-钙盐；提取人DPSCs，通过CCK-8实验研究不同浓度MDP-钙盐对于细胞增殖的影响，碱性磷酸酶（ALP）活力测试选取最适浓度，茜素红染色和实时荧光定量PCR研究MDP-钙盐对DPSCs成牙本质分化的影响。结果：一定浓度范围MDP-钙盐对DPSCs增殖无抑制作用，MDP-钙盐促进DPSCs的ALP活力的最佳浓度为0.2 mg/mL（P＜0.05），0.2 mg/mLMDP-2钙盐促进DPSCs钙结节形成（P＜0.05），0.2 mg/mL MDP-1钙盐和MDP-2钙盐对DPSCs中牙本质基质蛋白-1（DMP-1）的分泌有促进作用（P＜0.05），0.2 mg/mL MDP-0.5钙盐和MDP-1钙盐对DPSCs中牙本质涎磷蛋白（DSPP）的分泌有促进作用（P＜0.05），0.2 mg/mL MDP-0.5钙盐和MDP-2钙盐对DPSCs中骨钙素（OCN）的分泌有促进作用（P＜0.05），0.2 mg/mL MDP-0.5钙盐、MDP-1钙盐和MDP-2钙盐对DPSCs中Runt相关转录因子2（RUNX2）的分泌有促进作用（P＜0.05）。结论：MDP-钙盐在一定浓度范围内对DPSCs增殖无抑制作用，0.2 mg/mL MDP-钙盐对DPSCs成牙本质分化有促进作用。

关键词: 10-甲基丙烯酰氧癸二氢磷酸酯, 牙髓干细胞, 成牙本质分化

Abstract: Objective:?To investigate the effects of methacryloyloxydecyl dihydrogen phosphate (MDP)-calcium salts on the proliferation and odontogenic differentiation of dental pulp stem cells (DPSCs) and evaluate its role in the formation of tertiary dentin and the protection of dental pulp.Methods:?Three types of MDP-calcium salts were synthesized using different ratios of 10-MDP and CaCl2, and Human DPSCs were harvested and cultured in medium. The effect of different concentrations of MDP-calcium salts on cell proliferation was determined by CCK-8 assay.Alkaline phosphatase (ALP) activity was measured to screen the best concentration for subsequent experiments.Additionally, alizarin red staining, and real-time quantitative polymerase chain reaction (qRT-PCR) were employed to study the influence of a specific concentration of MDP-calcium salts on the odontogenic differentiation of DPSCs.Results:A certain concentration range of MDP-calcium saltshad no inhibitory effect on the proliferation of DPSCs. Compared with the control group, 0.2mg/mL was the optimal concentration of MDP-calcium salts in inducing ALP activity of DPSCs (P＜0.05). Alizarin red staining results showed that 0.2mg/mL MDP-2calcium salts promoted the formation of calcium nodules in DPSC0.2mg/mLMDP-1calcium salts and 0.2mg/mL MDP-2calcium salts promoted the secretion of odontogenic differentiation marker dental matrix protein-1 (DMP-1) (P＜0.05). Additionally, 0.2 mg/mL MDP-0.5calcium salts and 0.2 mg/mL MDP-1calcium saltsenhance the secretion of dentin sialophosphoprotein (DSPP) (P＜0.05). Furthermore, 0.2 mg/mL MDP-0.5calcium salts and 0.2 mg/Ml MDP-2calcium salts also enhanced the secretion of osteocalcin (OCN) (P＜0.05). Lastly, 0.2 mg/mL MDP-0.5calcium salts, 0.2 mg/mL MDP-1calcium salts and 0.2 mg/mL MDP-2calcium salts stimulated the secretion of runt-related transcription factor 2(RUNX2) (P＜0.05). Conclusions:?MDP-calcium salts can not inhibit the proliferation of DPSCs within a certain concentration range. 0.2mg/mLMDP-calcium salts can promote the odontogenic differentiation of DPSCs.

Key words: 10-methacryloyloxydecyl dihydrogen phosphate, dental pulp stem cell, Odontogenic differentiation

马语笛谢海峰. MDP-钙盐对牙髓干细胞增殖和成牙本质分化的影响[J]. 口腔生物医学, 2024, 15(5): 252-256.

Yu-Di MA. Effects of MDP-calcium salts on the proliferation and odontogenic differentiation of dental pulp stem cells[J]. Oral Biomedicine, 2024, 15(5): 252-256.

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