Abstract: [Abstract] Objective：To study the relationship between miR-200a expression and human ameloblastoma. Methods：RNAhybrid and miRanda software were applied to predict the β-catenin targeting miRNAs. 30 cases of human ameloblastoma and 5 cases of normal tooth germ tissues were selected to detect the expression of miR-200a by real-time PCR technology. Results：MiRNA targeting prediction showed that both the 68th to 96th base pairs and the 69th to 94th base pairs in β-catenin mRNA contained a miR-200a binding site. Compared with normal tooth germ tissues, the expression of miR-200a showed an obvious down-regulation in human ameloblastoma tissues, which difference was statistically significant (P < 0.05). Conclusions：There is a relationship between the expression of miR-200a with human ameloblastoma, and miR-200a might be one of the targeting gene of β-catenin.