Abstract: Objective：To construct specific miRNA expression vectors targeting membrane-type matrix metalloproteinase-1（MT1-MMP）gene and test the silent rates of four different vectors for MT1-MMP. Methods：Design four different specific oligo sequences of DNA targeting MT1-MMP, and then construct recombinant plasmids using BLOCK-iT? Pol II miR RNAi Expression Vector Kit with EmGFP. The positive ones were cloned. The transformed clones were sequenced and then amplified. The resultant correct plasmids were extracted and then transfected into the HEK293 cells by Lipofectamine 2000 to test the silent rates of MT1-MMP with real-time fluorogenic quantitative RT-PCR method．Results：The MT1-MMP miRNA interference vectors were constructed. The most effective interference vector was X173 - 4, and its target gene silencing efficiency can reach 75%. Conclusions：This study provides an experimental basis for further study on the role of MT1-MMP in invasion and metastasis of oral squamous cell carcinoma.