›› 2012, Vol. 3 ›› Issue (4): 189-192.

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The role SEMA3A and its receptor NRP-1 play in mediating the sprout of axons in co-cultures of SCC25 and rat’s DRG

  

  • Received:2012-10-16 Revised:2012-12-04 Online:2012-12-25 Published:2013-01-10

Abstract: [Abstract] Objective:To investigate the role semaphorin3A(SEMA3A) and its receptor neuropilin-1(NRP-1) play in the intriguing process that tumor grow without nervous tissue, we establish the model of cocutures of squamous cell carcinoma 25 (SCC25) and rat’s dorsal root ganglion(DRG). Methods: We tested the expression of SEMA3A and NRP-1 respectively in SCC25 and rat’s DRG. And then a model of cocutures of SCC25 and rat’s DRG was established. In the vitro cocultures, the difference of the DRG’s sprouting was observed when the medium treat with or without the antagonist of NRP-1. Results: Both the expression of SEMA3A in SCC25 and the expression of NRP-1 in rat’s DRG were positively detected, and the coculture showed that the rat’s DRG treating with the antagonist of NRP-1 sprouts better. Conclusions: SEMA3A and its receptor NRP-1 may play an important role in the process that tumor grow without nervous tissue.

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