Abstract: Objective: To construct overexpression vectors targeting rat Satb2 gene and study the transfection effect of Satb2 in bone marrow stromal cells(BMSCs). Methods: The Satb2 gene was insert into plasmid GV208 . The plasmid was detected by DNA sequencing. The recombined plasmid GV208-Satb2 was transfected into 293 T cell line. Virus yielded by 293T cell was transfected into rat BMSCs. The expression of Satb2 was detected by Western bolt. Results: The Satb2 was correctly cloned into the GV208 plasmid and confirmed by DNA sequencing. The result of Western blot showed that GV208-Satb2 significantly up-regulated the expression of Satb2 in rat BMSCs. Conclusions: Over-expression vector targeting rat Satb2 gene had been constructed successfully and efficiently up- regulated the expression of Satb2 in rat BMSCs.