›› 2013, Vol. 4 ›› Issue (3): 113-116.

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A preliminary study on the expression of Rap1b in zebrafish neural crest

  

  • Received:2013-07-09 Revised:2013-09-18 Online:2013-09-25 Published:2013-10-22
  • Contact: Lei YU E-mail:642201683@qq.com

Abstract: Objective:To find the relationship between Rap1b and the zebrafish craniolfacial development. Methods:Detect the expression of Rap1b in the head of zebrafish which was derived from neural crest by real-time RT-PCR.Then compare the expression of Rap1b and Crestinin the process of zebrafish development with in situ hybridization. Results:The results of real-time RT-PCR indicated that Rap1b was expressed in the craniofacial of zebrafish during the neural crest induction, migration and derivation. The results of in situ hybridization indicated that the expression of Rap1b and Crestin were partial overlap in the dimention of time and space of zebrafish develpment. Conclusions:Rap1b may influence the development of zebrafish craniofacial by regulating the development of neural crest.