›› 2015, Vol. 6 ›› Issue (2): 95-99.

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Effects of two fimA variants of Porphyromonas gingivalis on the function of human umbilical artery smooth muscle cells

  

  • Received:2015-03-26 Revised:2015-05-21 Online:2015-06-25 Published:2015-07-13

Abstract: Objective:To explore effects of viability on human umbilical artery smooth muscle cells affected by two different fimAgenetypes of Porphyromonas gingivalis. Methods:The tissue-block attached method was used for primary culture of human umbilical artery smooth muscle cells. Porphyromonas gingivalis was cultured in the anaerobic conditions. Human umbilical artery smooth muscle cells were invaded by fimA genotype Ⅰ and Ⅳstrains respectively for 2, 8, 24 and 48 h. The CCK-8 assay was applied in testing viability of smooth muscle cells. Results: After fimA typeⅠstrains were cultured with vascular smooth muscle cells, smooth muscle cells were significantly increasing at 8 h, but obviously, cytotoxicity appeared at 24 h and 48 h (P<0.05). Cells invaded by fimA type Ⅳ Porphyromonas gingivalis did not have any significant change as compared with that of control group. Conclusions:There are diversities between the two different strains. FimA type Ⅰ strain plays a role in the processes of facilitating proliferation and cytotoxicity of vascular smooth muscle cells.