›› 2015, Vol. 6 ›› Issue (2): 62-65.

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Functional annotation clustering of genes in mouse tongue myogenesis

  

  1. 1. Dalian Medical University
    2. 大连医科大学 口腔医学院 口腔基础教研室
    3. Department of Oral Basic Science, College of Stomatology, Dalian Medical University
  • Received:2015-03-27 Revised:2015-05-17 Online:2015-06-25 Published:2015-07-13

Abstract: Objective:To gain insight into the molecular mechanisms associated with mouse tongue myogenesis. Methods:Different genes in the tongue at mouse embryonic day 13.25 (E13.25) and 15.5 was investigated using Affymetrix Mouse GeneChip. Using the twice significance of difference as the standard, the molecular mechanisms of tongue development were studied and several molecules related were identified by DAVID functional annotation clustering analysis. Results:Genes of higher expression level at E13.25 were related to cell cycle and cell adhesion, of whom Exo1, Gsk3B, Kif20b, Skp2 ( cell cycle related factors) and Neo1 and lama1 (cell adhesion factors) were activated. While genes of higher expression level at E15.5 were related to cytoskeleton, such as titin and Hspb7. Conclusions:The proliferation and determination of tongue were related with gene clusters of cell cycle and cell adhesion, and, differentiation and maturation of tongue were relevant to gene cluster of cytoskeleton. It had highlighted potential cascades and important candidates for further investigation on the genetic mechanism and clinical therapy of tongue related diseases.