›› 2015, Vol. 6 ›› Issue (2): 87-89.

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Effect of cryopreservation on proliferation and differentiation of periodontal ligament stem cell sheet

  

  • Received:2015-03-27 Revised:2015-06-03 Online:2015-06-25 Published:2015-07-13

Abstract: Objective:To investigate the effect of cryopreservation on proliferation and differentiation of periodontal ligament stem cells (PDLSCs) sheet. Methods:We compared cryopreserved PDLSCs sheet with freshly PDLSCs sheet in terms of cell proliferation rate and multilineage differentiation potential by MTT, von Kossa staining and Oil Red staining. Results:Cryopreserved PDLSCs sheet showed a similar cell proliferation rate and multilineage differentiation potential as fresh PDLSCs sheet. Conclusions:This study demonstrates that cryopreservation does not affect the proliferation and differentiation of PDLSCs sheet, supporting the feasibility of PDLSCs sheet cryopreservation in nitrogen.