Abstract: Objective: To investigate the effect of advanced glycation end products ( AGEs) on the adipogenic differentiation of human periodontal ligament stem cells ( HPDLSCs) and detect the C/EBPβ、PPAR-γ gene expression．Methods: HPDLSCs were isolated by limited dilution of culture cells for single cell clone, The osteogenic differentiation capacity of HPDLSCs was evaluated by Alizarin red staining, the adipogenic differentiation capacity of HPDLSCs was evaluated by oil red staining. HPDLSCs were induced with AGEs, Real time quantitative reverse transcription polymerase chain reaction (real time PCR) was performed to detect the differences of gene expression between the control group and experimental group. Results:After 21 days induction，Alizarin red staining showed mineralization nodules were formation, oil red staining showed lipid droplets were formation. After AGEs stimulated,during HPDLSCs adipogenic differentiation,the formation of lipid droplets increased in the experimental group, the expressions of CCAAT enhancer binding protein β(C/EBP β)、peroxisome proliferator activated receptorsγ (PPAR-γ) in the experimental group were higher than those in the control group,the differences were statistically significant (p<0.05). Conclusions:AGEs promote the adipogenic differentiation capacity of HPDLSCs,and changed the expressions of C/EBP-β、PPAR-γ mRNA levels.