›› 2015, Vol. 6 ›› Issue (4): 172-177.

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Research on the relationship between O-GlcNAc and oral squamous cell carcinoma

  

  • Received:2015-10-29 Revised:2015-11-24 Online:2015-12-25 Published:2015-12-30

Abstract: Objective:To investigate the relationship between O-linked N-acetylglucosamine (O-GlcNAc) and oral squamous cell carcinoma. Methods:The expression of O-GlcNAc, O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA) in 30 cases of oral squamous cell carcinoma and 10 normal oral mucosa were detected using immunohistochemical staining. The proliferation of TCA8113 with transglycosylation (TG) and deoxynorleucine (DON) was examined by Immunofluence and Western blotting. Results:There was significant difference of O-GlcNAc and OGT between normal mucosa and oral squamous cell carcinoma (P<0.05). The expression of O-GlcNAc and OGT increased with higher grade of the carcinoma. The expression of OGA was inconsistent with O-GlcNAc and OGT. TG could activate the expression of O-GlcNAc and OGT, DON could inhibit the expression of O-GlcNAc and OGT. Besides, DON could inhibit the proliferation of TCA8113 cells and the expression of PCNA. Conclusions:O-GlcNAc could activate the oral squamous cell carcinoma. Inhibitor DON could depress the proliferation of TCA8113.