›› 2015, Vol. 6 ›› Issue (4): 198-210.

Previous Articles     Next Articles

Exogenous inhibition of Neuropilin-1 effects on the migration and the differentiation of human tongue squamous cell carcinoma cell line SCC9

  

  • Received:2015-06-23 Revised:2015-08-13 Online:2015-12-25 Published:2015-12-30

Abstract: Objective: To investigate the effect exogenous inhibition of Neuropilin-1(NRP-1) on the migration and the differentiation of the human tongue squamous carcinoma cell line SCC9. Methods: Western blot were performed to examine the expression of NRP-1 in cell line SCC9 and the human tongue squamous carcinoma cell cancer tissue. Besides, scratch test and transwell assay were employed to show the influence of exogenous inhibition of Neuropilin-1(NRP-1)on the migration of the SCC9 cells. And Real-time RT PCR were used to detect the expression of differentiation markers in the SCC9 cells exogenously inhibited of NRP-1. Results: NRP-1 express at high level in the SCC9 cells, and exogenous inhibition of NRP-1 depressed the migration of SCC9 cells. Real-time RT PCR showd the expression of epithelial cadherin and β-catenin increased, while the expression of snail, fibronectin and α-smooth muscle actin decreased. Conclusions: Exogenous inhibition of NRP-1 depressed the migration and differentiation of SCC9 cells.

CLC Number: