›› 2016, Vol. 7 ›› Issue (1): 1-5.

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ClC-3 chloride channel in the regulation of TGF-β pathway on the osteogenic differentiation effect of PTH

  

  • Received:2015-11-20 Revised:2015-12-19 Online:2016-03-25 Published:2016-04-06

Abstract: Objective:To investigate the mechanism of ClC-3 chloride channel in the progress of PTH regulating bone osteogenic differentiation. Methods:Regulate the expression of TGF-β1 and ClC-3 chloride channel in MC3T3-E1 cells with specific siRNA. Investigate the expression of Clcn3、Tgfb1 and osteogenic factors (Alp, Bsp, Oc and Runx2) with the method of Real-time PCR. Investigate the expression of CLC-3 chloride channel protein and TGF-β1 protein with the method of Western Blot and immunofluorescence. Results: The effect of TGF-β1 gene expression on ClC-3 expression in osteoblasts under discontinuous stimulation of PTH: ClC-3 chloride channel gene and protein expression increased when TGF-β1 gene expression in osteoblasts was interrupted only. The effect of ClC-3 chloride channel gene expression on TGF-β1 expression in osteoblasts under discontinuous stimulation of PTH: TGF-β1 gene and protein expression increased when ClC-3 chloride channel gene expression was interrupted only. Expression of osteogenic genes under discontinuous stimulation of PTH: Expression of osteogenic genes (Alp, Bsp, Oc and Runx2) decreased comparing to control when ClC-3 gene and TGF-β1 gene were interrupted respectively (P<0.05). However, no significant difference was observed between the expression of the simultaneously interrupted genes and the control (P>0.05). Conclusion:ClC-3 chloride channel has been involved in the osteoinduction of PTH in the osteoblasts through the TGF-β pathway. The specific mechanism behind that requires further research.

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