Abstract: The purpose of this study was to discuss the correlation of autophagy and angiogenic capacity in co-culture using human amniotic membrane-derived mesenchymal stem cells (hAMSCs) with human umbilical vein endothelial cells (hUVECs) in 3D gels by comparing to hUVECs-monoculture. Methods：To isolate hAMSCs and hUVECs, Amnion were incubated with dispase and collagenase, while umbilical veins were digested with collagenase type 1. Identification of hAMSCs and hUVECs through Flow Cytometry , immunofluorescence staining and Multilineage differentiation. The length of sprouting and average vessel area were surveyed in hUVEC-hAMSC co-culture and hUVECs-monoculture at 0, 3, 6, 12, 24, 48, 72h. ATG5, Beclin-1 and LC3Ⅱ was analyzed in hUVEC-hAMSC co-culture and hUVECs-monoculture by western blotting a 0, 3, 6, 12, 24, 48, 72h. Results：hAMSCs were positive for MSC markers, while negative for hematopoietic and vascular cellrelated markers. hUVECs were positive for CD34 and negative for CD45. hUVECs expressed CD31 on membrane surface and vWF in cytoplasm. The length of sprouting and average vessel area in hUVEC-hAMSC co-culture were more than those in hUVECs-monoculture after 12 h. Expression of ATG5 in hUVEC-hAMSC co-culture increased significantly in hUVECs –monoculture at 3 and 6 h, while Beclin 1 and LC3Ⅱ rose remarkably in hUVEC-hAMSC co-culture before 6 h and 12h respectively. Conclusions：In conclusion, this study discovered that hAMSCs can promote angiogenic capacity and demonstrated that the capacity of promote angiogenic had intimate connection with elevated level of intracellular autophagy at early co-culture system.