›› 2016, Vol. 7 ›› Issue (2): 67-71.

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TNF-α inhibits mBMSCs osteoporosis through miR-21

  

  • Received:2016-04-07 Revised:2016-05-25 Online:2016-06-25 Published:2016-06-22

Abstract: Objective:To investigate whether or not the TNF-α can affect mouse bone marrow stromal stem cells (mBMSCs)osteoporosis through miR-21.Methods:Real-time RT-PCR was used to detect the levels of miR-21 in mBMSCs osteogenesis on condition of TNF-α. Osteogenic gene and protein was determined by real-time RT-PCR and Western blot analysis, respectively. After upregulation the of miR-21 in the presence of TNF-α, the osteogenic potential of mBMSCs were investigated. Results:TNF-α inhibits mBMSCs osteoporosis and miR-21 can restore the potential of mBMSCs treated with TNF-α. Conclusions:TNF-α inhibits mBMSCs osteoporosis through miR-21.