›› 2016, Vol. 7 ›› Issue (4): 183-186.

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Investigation of miR-223 on the of RANKL in human periodontal ligament fibroblasts cells

  

  • Received:2016-12-08 Revised:2016-12-23 Online:2016-12-25 Published:2017-01-09
  • Contact: Mi-Fang Yang E-mail:yangmifang@qq.com

Abstract: Objective: Investigate the role of miR-223 on the of RANKL in HPDLFs. Methods:The HPDLFs over expressed with miR-223 was constructed, the of RANKL and OPG were detected by western blot and real time PCR, and the change of RANKL/OPG ratio was calculated. Results: The RANKL was down-regulated in the HPDLFs with miR-223 over expressed and the RANKL/OPG ratio was decreased. Conclusions: MiR-223 could disrupt the homeostasis of RANKL/OPG, destroy the microenvironment of osteoclast differentiation. MiR-223 may play a regulatory role in osteoclast differentiation .