碳酸锶/丝素蛋白纳米纤维膜的构建及其对骨髓间充质干细胞增殖分化的影响

Abstract

Abstract: Objective：The aim of the study was to fabricate the novel silk fibroin/strontium carbonate nanofibrous membranes (SrCO3-SFM) and explore the effects on the bioactivity of BMSCs. Methods：We fabricated the SrCO3-SFM by electrospinning and biomineralization process. SEM and XRD were used to determine the structure characteristics of SrCO3-SFM. To examine the biocompatibility of SrCO3-SFM, we investigated cell proliferation and differentiation by cck-8, ALP and RT-PCR. Results：The results showed lots of SrCO3 particles, which evenly distributed on the nanofibers. In vitro tests, the SrCO3-SFM promoted the activity of ALP and extracellular matrix mineralization and increased the of osteogenic differentiation related genes, such as RUNX2, BSP and OCN. Conclusions：The SrCO3-SFM material has good cell compatibility, promoting mesenchymal stem cell proliferation and osteogenic differentiation.

CLC Number:

R783.1

References

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Effects of the novel silk fibroin/strontium carbonate nanofibrous membrane on the bioactivity of bone marrow mesenchymal stem cell

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