›› 2017, Vol. 8 ›› Issue (2): 95-99.

Previous Articles     Next Articles

Effect of platelet rich fibrin membrane on periodontal healing of delayed replanted rat tooth

  

  • Received:2017-03-31 Revised:2017-06-13 Online:2017-06-25 Published:2017-07-03

Abstract: Objectives:To investigate the effect of platelet rich fibrin (PRF) membrane on periodontal healing of delayed replanted avulsed teeth. Methods:Twenty-five six-week-old male Wistar rats were used for split mouth design. After bilateral maxillary first molars extracted, dried for 30 min, self-made PRF membrane was placed in one side of the extraction socket and the avulsed teeth replanted as PRF membrane group, without PRF membrane at contra-lateral, avulsed teeth replanted as control group. On 1, 3, 7, 14 and 28 days after tooth replantation, the rats were sacrificed. Paraffin specimens of maxillary bone were prepared, sliced. HE staining was used to observe the periodontal healing of the replanted teeth and the results were statistically analyzed. Results:All the replanted teeth did not fall off. One d after tooth replantation, inflammatory infiltrate could be found in both groups, the incidence rate of periodontal inflammatory change was significantly higher in PRF membrane group than that of control group (P<0.01); After 3 days, the incidence rate of periodontal inflammatory change was still higher in PRF membrane group (P<0.01); After 7 days, the incidence rate of periodontal membrane healing was consistent in both groups(P>0.05), incidence rate of surface resorption was significantly lower in PRF membrane group than in control group (P<0.05), inflammatory resorption was consistent in both groups(P>0.05),14 days after operation, incidence rate of surface resorption was significantly higher in PRF membrane group than that of control group (P<0.05) while inflammatory resorption was significantly lower in PRF membrane group (P<0.01); 28 days after operation, periodontal healing pattern in the two groups were similar. Conclusions:In delayed avulsion teeth replantation, the application of PRF membrane did not affect the reposition and healing of replanted teeth, and could control the occurrence and development of root resorption at the initial stage. However, the long-term effect is not stable, and needs further study.