Abstract: Objective：To analyze the effect of miR-495-3p on the proliferation of hPDLC and the production of inflammatory cytokines that induced by LPS. Methods：The primary hPDLC was isolated and was treated with LPS or LPS+miR-495-3p mimic transfection. The of miR-495-3p and TLR4 was measured by Real-time PCR. The proliferation of hPDLC was measured by MTT method. The production of IL-6 and TNF-α was measured by ELISA. The target relationship between miR-495-3p and TLR4 was measured by dual luciferase assay. The of TLR4 and IκBa was measured by Western blot. Results: LPS significantly down-regulated the of miR-495-3p and up-regulated the of TLR4. MiR-495-3p mimic dramatically increased the proliferation of hPDLC, decreased the production of IL-6 and TNF-α. MiR-495-3p targeted the 3' UTR region of TLR4. MiR-495-3p mimic significantly decreased the of TLR4. Furthermore, miR-495-3p mimic noticeably suppressed the of p-IκBα. Conclusions：miR-495-3p increased the proliferation of hPDLC and suppressed the production of inflammatory cytokines through down regulated the of TLR4 and inhibited NF-κB pathway.