Abstract: [Abstract]Objective: Polycaprolactone/type I collagen/fluoroapatite composite scaffolds were prepared for periodontal tissue regeneration and biocompatibility and osteogenic induction were tested. Methods: Polycaprolactone/type I collagen electrospun nanofiber membrane was prepared, and the fluoroapatite mineralized coating was synthesized on the surface. The morphology was observed by scanning electron microscopy. At the same time, human periodontal ligament cells were isolated and cultured on the surface of the scaffold, and the cell proliferation was detected by CCK-8 method. The effects of composite scaffold on osteogenic differentiation of human periodontal ligament cells were observed by alkaline phosphatase staining, alizarin red staining and Von Kossa staining.Results: The polycaprolactone/type I collagen/fluoroapatite composite scaffold showed a three-dimensional network structure, and the fluoroapatite crystals were distributed on the surface of the scaffold. The CCK-8 results showed that the human periodontal ligament cells proliferated well on the composite scaffold. Alkaline phosphatase staining, alizarin red staining and Von Kossa staining results suggest that the composite scaffold can induce osteogenic differentiation of human periodontal ligament cells. Conclusion: The polycaprolactone/type I collagen/fluoroapatite composite scaffold has good biocompatibility and osteogenic induction potential, and it is expected to be used as a scaffold material for periodontal tissue engineering.