Abstract: Objective： To study the effect of secreted frizzled-related protein 2 (SFRP2) on lymph node T cells in C57 mice. Methods： SFRP2 recombinant protein was used to construct high concentration SFRP2. The total T cells of lymph node from C57 mouse were isolated and cultured in vitro. The effects of SFRP2 recombinant protein on proliferation, apoptosis and subpopulation of lymph node T cells in C57 mice were detected by flow cytometry. Results： SFRP2 (0.4 ng/mL) inhibited the proliferation of total T cells;SFRP2 (0.4 ng/mL)inhibited the early apoptosis of total T cells, has no significant effect on late apoptosis rate, and inhibits total apoptosis rate; SFRP2 (0.4 ng/mL) increases the proportion of Th1 subpopulations, decreases the proportion of Th2 subpopulations, and changes the Th1/Th2 balance. Conclusions： The results indicate that the concentration of SFRP2 (0.4 ng/mL) can inhibit the proliferation of total T cells, promote apoptosis, and affect the balance of Th1/Th2.

Key words: secreted frizzled-related protein 2 , total T cell, Th1/Th2balance