›› 2020, Vol. 11 ›› Issue (1): 45-48.

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A basic study on the application of gold immunochromatographic assay based on il-1 in GCF detection of periodontitis

  

  • Received:2019-12-31 Revised:2020-02-08 Online:2020-03-25 Published:2020-04-07

Abstract: Objective: To develop a colloidal gold-based immunochromatographic test strip for the rapid detection of IL-1β levels in the gingival crevicular fluid (GCF), expecting to provide experimental evidence for the accurate diagnosis of chronic periodontitis. Methods: The colloidal gold was prepared by chloroauric acid and trisodium citrate. After optimizing the reaction conditions, the colloidal gold-antibody complex was prepared. Then, with the assembled colloidal gold chromatography test strips, the standard curve was tested and the clinical samples were detected, whose results were analyzed by Spearman correlation with those of ELISA. Results: The colloidal gold immunochromatographic test strips were successfully prepared, and the detection sensitivity could reach 5 ng/mL. The clinical samples detection showed that the Spearman correlation coefficient between the test strips and ELISA was 0.992 for detecting IL-1β levels in GCF in periodontitis patients. Conclusion: The developed colloidal gold-based immunochromatographic test strips can perform reliable detections, which provide experimental evidence for rapid detection of IL-1β levels in GCF in periodontitis patients.

Key words: IL-1β, Point-of-Care Testing, GNPs, lateral flow strips.