Abstract: Objective: To investigate the effect of titanium nanotubes formed by electrochemical anodization, loaded with genipin crosslinked chitosan hydrogel on biological activity of mouse bone marrow mesenchymal stem cells (BMSC) in vitro. Methods:?A two-step electrochemical anodization technique was used to prepare a regular array of titanium dioxide nanotubes on the substrate of titanium, chitosan coating was loaded by negative pressure method, and crosslinked with genipin. Field emission scanning electron microscope was used to observe the morphology of different substrates, and the contact angle measuring instrument was used to measure the hydrophilicity. The samples were co-cultured with BMSC, and cell adhesion and proliferation were measured by the CCK-8. Results:?Anodic oxidation method and negative pressure method was used to construct composite nano-structures. Field emission scanning electron microscopy showed particles on the surface, which showed increased hydrophilicity. The results of cell experiments confirmed that the nanocomposite had no obvious effect on the adhesion of BMSC, but promoted cell proliferation. Conclusions: Nanocomposites of titanium dioxide nanotubes loaded with genipin crosslinked chitosan hydrogel can improve BMSC activity.

Key words: nanotubes, genipin, chitosan, bone marrow stromal cells, proliferation