Abstract: Objective:To collect and analyze the whole genome transcription information of gingival tissue of patients with impacted maxillary canine, and lay a foundation for the gene theory of the etiology. Methods:Using resequecing Illumina hiseq2000 sequencing platform, the transcriptome information of gingival tissue of 5 adult patients (25-35 years old) with impacted maxillary canines in Zunyi area was collected by high-throughput sequencing. The expression level and difference of gene in gingival tissue were analyzed and calculated by FPKM method. Results:223530890 high quality sequencing data were obtained, and 19352 genes were indentified to be expressed in the gingival tissue, among which 99.63% genes’ FPKM values were lower than 1000. 15412 genes had alternative splicing (AS), and the aplicing times were 16167. Mutually exclusive exons were found to be the rarest AS type (6.72%). While skipped exons found to be the major type (48.82%). Using these transcriptome data, 386414 SNPs were called, among which transitions were the main type (76.31%). According to GO database, transcriptome SNPs were involved in a wide range of biological functions, and are especially enriched in biological processes and cell components; KEGG pathway analysis shows that the genes of SNPs are enriched in metabolic pathways, endocytosis, apoptosis, cell aging and other related pathways, and more genes are enriched in cancer, signaling pathways and immune system related pathways. Conclusions:Genome transcription information of gingival tissue of patients with impacted maxillary canine showed gingiva were closely related to the development of teeth.

Key words:

single nucleotide polymorphism, ?gingival tissue, ?transcriptome, ?periodontal tissue