Abstract: Objective:?To investigate the effect of miR-206-3p on osteoclastogenesis. Methods:?RAW264.7 cells were induced into osteoclasts in vitro. The knockdown and over expression efficiencies of miR-206-3p were examined by real-time fluorescence quantitative PCR (qRT-PCR). Then F-actin staining and trap-resistant acid phosphatase (TRAP) staining were performed to detect the osteoclastogenesis, and the expression of osteoclast differentiation-related gene NFATc1 was evaluated by qRT-PCR and Western Blot. Results:?The overexpression of miR-206-3p significantly inhibited osteoclastogenesis and the expression of NFATc1, while the inhibition of miR-206-3p did the opposite effect (P<0.05). Conclusions:?miR-206-3p regulates the differentiation of RAW264.7 cells into osteoclasts by targeting the expression of NFATc1.