干扰MT1-MMP表达对口腔鳞癌细胞增殖、侵袭的影响

Abstract

Abstract: Objective: To detect the expression of membrane type 1 matrix metalloproteinase (MT1-MMP) in oral squamous cell carcinoma (OSCC) and its role on the proliferation and invasive ability of OSCC. Methods: RT-PCR was used to detect the expression of MT1-MMP and E-cadherin in OSCC and adjacent tissues; Small interfering RNA (siRNA) was used in targeted interference MT1-MMP gene in HSC3 cells. The experiment was divided into four groups: siControl group, siMT1-MMP group, siControl+transforming growth factor β1 (TGF-β1) group, siMT1-MMP+TGF-β1 group. CCK-8 method was used to detect cell proliferation of HSC3. Transwell assay was used to detect the invasive ability of cells. The expression of MT1-MMP, E-cadherin, TGF-β signaling pathway related proteins (CUTL1 and Wnt5a) was detected by Western blot. Results: Compared with the adjacent tissues, higher expression of MT1-MMP and lower expression of E-cadherin was exhibited in OSCC（P<0.05）; the cell proliferation of HSC3 cells did not change significantly after MT1-MMP interference（P＞0.05）; compared with siControl group and siMT1-MMP group, the HSC3 cell invasion number in siControl+TGF-β1 group and siMT1-MMP+TGF-β1 group was increased significantly; compared with siControl+TGF-β1 group, the HSC3 cell invasion number in siMT1-MMP+TGF-β1 group was decreased significantly（P<0.01）; compared with siControl group, protein expression of MT1-MMP, CUTL1 and Wnt5a increased significantly in siControl+TGF-β1 group, while the expression of E-cadherin decreased（P<0.01）; compared with siControl+TGF-β1 group, protein expression of MT1-MMP, CUTL1 and Wnt5a decreased partially in siMT1-MMP+TGF-β1 group, while the expression of E-cadherin increased significantly（P<0.05）. Conclusions: MT1-MMP interference may inhibit the invasion of OSCC through regulating the EMT process induced by TGF-β1.

Key words: oral squamous cell carcinoma, membrane type 1 matrix metalloproteinase, E-cadherin, epithelial-mesenchymal transition

References

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Effects of MT1-MMP interference on the proliferation and invasive ability of oral squamous cell carcinoma

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