Oral Biomedicine ›› 2022, Vol. 13 ›› Issue (3): 148-153.

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VEGF promotes the involvement of endothelial progenitor cells in periodontal tissue remodeling in rat orthodontic experimental model

  

  • Received:2022-03-08 Revised:2022-07-17 Online:2022-09-25 Published:2022-09-29

Abstract: Objective:To observe the distribution of vascular endothelial progenitor cell (EPC) in the periodontal tissue remodeling area of orthodontic experimental rats and to investigate the effect of vascular endothelial cell factor (VEGF) on their chemotaxis and differentiation. Methods:Experimental rat tooth movement model was established, and rat peripheral blood EPC were isolated, cultured and identified. The proliferation and migration of EPC were observed by intervening VEGF in vitro. BrdU-labeled EPC were injected into model rats through the tail vein to observe the distribution of EPC in periodontal tissues, and the expression of VEGF. Results:Cell phenotype identification was completed by isolating and culturing rat EPC. VEGF can promote the proliferation ability of EPC in vitro and promote the chemotaxis and migration of EPC. After injection of BrdU-labeled EPC into model rats, the number of BrdU EPC positive periodontal tissues increased; the expression of VEGF on the tension side of the experimental group (tail vein injection of EPC cells) was higher than that of the control group (injection of saline). Conclusions:During orthodontic tooth movement, peripheral blood EPC can be chemotactic and accumulate in periodontal tissue, and participate in periodontal tissue remodeling under the regulation of VEGF.

Key words: orthodontic tooth movement, vascular endothelial progenitor cells, angiogenesis, vascular endothelial cell growth factor