Has_circ_0008717在羊膜间充质干细胞上清促血管生成中的作用研究

Abstract

Abstract: Objective:?To investigate the role of has_circ_0008717 during the process that human amnion-derived mesenchymal stem cells (hAMSCs) promote angiogenesis of human umbilical vein endothelial cells (HUVECs). Methods:?Wound healing assay, Transwell migration and tube formation were used to confirm the pro-angiogenic capacity of conditioned medium from hAMSCs (hAMSC-CM) on HUVECs, qRT-PCR and were used to detect the change of level of the potential pro-angiogenic relative circRNAs and genes in HUVECs after treated with hAMSC-CM, among them, has_circ_0008717 and VEGFA attracted our attention. siRNAs specially targeted has_circ_0008717 were used to down-regulate the level of has_circ_0008717 in HUVECs treated with hAMSC-CM, and then qRT-PCR was used to detect were used to evaluate the change of level of VEGFA caused by down-regulation of has_circ_00087, afterwards,wound healing assay, Transwell migration and tube formation were used to assess the effects of down-regulation of has_circ_0008717 on HUVECs treated with hAMSC-CM. Results: Enhanced migration and tube formation in HUVECs induced by hAMSC-CM were observed (P＜0.05). And has_circ_0008717 been the mostly up-regulated circRNA among several potential pro-angiogenic relative circRNAs was selected for further investigation (P＜0.05). Increased expression of vascular endothelial growth factor A (VEGFA) was observed among a few pro-angiogenic relative genes (P＜0.05). Furthermore, these can be inhibited by silencing has_circ_0008717 in HUVEC treated with hAMSC-CM (P＜0.05). Conclusion: has_circ_0008717 might be involved in the pro-angiogenic role of hAMSC-CM in HUVECs.

Key words: circular RNA ABCB10, hAMSCs, angiogenesis, VEGFA

References 35

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The function of has_circ_0008717 in pro-angiogenic capacity of conditioned medium from human amnion-derived mesenchymal stem cells

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