Objective： To investigate the of nuclear matrix protein- special AT-rich sequence binding protein 2 (SATB2) in oral squamous cell carcinoma (OSCC) and its effect on the biological behavior of oral squamous cell carcinoma. Methods：58 cases of oral squamous cell carcinoma were collected and the of SATB2 was detected by immunohistochemical staining. The relationship between SATB2 and the pathological parameters and the overall survival rate was analyzed. Then the HN6 cell lines were transfected with lentivirus containing SATB2 and tested through western blot and RT-PCR as well. Wound healing test,migration tests and invasion tests were preformed for further study of biological roles of SATB2 in HN6 cell lines. Results：The high of SATB2 was not associated with age, sex and pathological grade, but was closely related to TNM stage, cervical lymph node metastasis and clinical stage, which was closely related to the poor prognosis. The results of Western blot and PCR showed that SATB2 was successfully transfected into HN6 cell line and Lv-SATB2-HN6 cells stably overexpressing SATB2 were obtained. The HN6 cells overexpressing SATB2 migrated and the invasive ability was significantly higher than that of HN6 cells. Conclusion：The of SATB2 in oral squamous cell carcinoma and cell line increased, and over of SATB2 could promote the migration and invasion of tumor cells.

Objective：To investigate the effect of let-7c on the proliferation and odonto/osteogenesis differentiation of rat bone marrow mesenchymal stem cells in vitro. Methods：Rno-let-7c over / low lentiviral vector were constructed and used to transfect rat BMMSCs in vitro, then the optimal transfection titer was screened. The of green fluorescent protein was observed by inverted fluorescence microscope. Real-time quantitative RT-PCR was used to verify the efficiency of rno-let-7c transfection. The effect of rno-let-7c on rBMMSCs proliferation and apoptosis was detected by CCK-8 and Flow Cytometry. The of insulin-like growth factor 1 receptor (IGF-1R) and odonto/osteogenic markers (RUNX2/OSX/OCN/DMP1) were detected by western blot. Results： The optimal condition of lentiviral vector to transfect rBMMSCs was MOI=3. Rno-let-7c over/low BMMSCs model was constructed successfully. Rno-let-7c over/low had no significant effect on proliferation and apoptosis of rat BMMSCs (P>0.05). Compared with the negative transfection group, the of IGF-1R and odonto/osteogenic markers was down-regulated in over group, while the of IGF-1R and odonto/osteogenic markers was up-regulated in low group (P>0.05). Conclusions：Let-7c had no obvious effect on the proliferation and apoptosis of rat BMMSCs. Let-7c can inhibit the odonto/osteogenic differentiation of rat BMMSCs.

Objective：To establish a three-dimensional co-culture model of oral cancer cells/fibroblasts and provide a new research method for the follow-up study on the invasion and metastasis of oral cancer. Methods：The method of tissue block was used to isolate and cultivate primary oral mucosa of normal fibroblasts (NFs). NFs and oral cancer cells (Cal27) were used to construct the three-dimensional co-culture model. HE and immunofluorescence staining were used to observe the growth and relationship between Cal27 and NFs of the co-culture model. Results：NFs were successfully harvested by primary culture. Using a floating culture for 4 days, the collagen gel contracted and Cal27 cells showed monolayer growth. When the collagen gel was transferred to the air-liquid interface culture conditions, Cal27 cells showed multilayer growth after 3 days, and the tumor cells exhibited a basement membrane like structure. After 9 days, the basement membrane like structure grew integrated. Conclusions：The three-dimensional air-liquid interface culture model is different from two-dimensional cell culture, which is similar to human tissue structure to form the oral epithelium and the underlying stromal cell layer, so that the oral cancer cells more resemble the human tissue in the growth pattern. Thus it provides a new method and approach for the study of oral cancer.

Objective：To investigate of special AT-rich sequence-binding protein 2（SATB2） in oral squamous cell carcinoma （OSCC）tissue samples，and then to study the proliferation roles of SATB2 in OSCC cells. Methods：The of SATB2 in OSCC and HN4 cell lines are tested by Western blot and qRT-PCR, cellular immunofluorescence was performed to further characterize the subcellular distribution of SATB2 in HN4 cell lines． Using lentivirus overexpressed SATB2, in vitro, cell proliferation were assessed by CCK-8 experiments and the cell cycles were measured by flow cytometry, the protein change of cell cycle-related protein P63, Cyclin B1 and STAT3 phosphorylation were tasted by Western blot. In vivo, the growth of tumor transplation with HN4 cells and Lv-SATB2-HN4 cells in nude mice was further observed . Results：The of SATB2 in OSCC and HN4 cell lines are tested by Western blot and qRT-PCR, and of SATB2 in OSCC tissues is higher than para-carcinoma tissues and of SATB2 in HN4 cells is higher than the oral keratin forms cells （P＜0.05）. CCK-8 assay, flow cytometry and xenograft model showed that over SATB2 in HN4 cell lines significantly promoted cell proliferation, cell cycle-related protein P63，Cyclin B1 had been significantly up-regulated and STAT3 phosphorylation obviously enhanced．Conclusions：SATB2 was highly expressed both in OSCC tissues and HN4 cell lines. Either in vitro or in vivo , over of SATB2 promoted cell proliferation.

Objective：To discuss the research status of dental stem cells. Methods Information was retrieved in database of Web of Science with retrieval type SU=dental stem cell. 3221 records were observed. Analysis was done on the top 10 countries,institutions and authors by bibliometric and visualized by CiteSpace. The hot spots and frontier evolution were observed by co-keyword analysis and co-citation analysis. Results： The number of papers continues to rise since 1999. The No 1 top country is USA, No 1 top institution is the Fourth Military Medical University of China, No 1 author is SHI ST from America. The first keywords of high frequence in the recent 5 years are: immature permanent teeth, exfoliated deciduous teeth, human periodontal ligament, osteogenic differentiation, mineral trioxide aggregate, et al. Conclusions：Research on dental stem cells is a hot point of oral basic study in recent years. Researchers focused on pulp, teeth, bone regeneration with dental pulp stem cells, human periodontal ligament, dental follicles stem cells, et al. CiteSpace can help to ravel out the subject.

Objective：To analyze the effect of miR-495-3p on the proliferation of hPDLC and the production of inflammatory cytokines that induced by LPS. Methods：The primary hPDLC was isolated and was treated with LPS or LPS+miR-495-3p mimic transfection. The of miR-495-3p and TLR4 was measured by Real-time PCR. The proliferation of hPDLC was measured by MTT method. The production of IL-6 and TNF-α was measured by ELISA. The target relationship between miR-495-3p and TLR4 was measured by dual luciferase assay. The of TLR4 and IκBa was measured by Western blot. Results: LPS significantly down-regulated the of miR-495-3p and up-regulated the of TLR4. MiR-495-3p mimic dramatically increased the proliferation of hPDLC, decreased the production of IL-6 and TNF-α. MiR-495-3p targeted the 3' UTR region of TLR4. MiR-495-3p mimic significantly decreased the of TLR4. Furthermore, miR-495-3p mimic noticeably suppressed the of p-IκBα. Conclusions：miR-495-3p increased the proliferation of hPDLC and suppressed the production of inflammatory cytokines through down regulated the of TLR4 and inhibited NF-κB pathway.

Objective：To investigate the effect of the hydroxyl safflor yellow A in regulating the stemness and the activity of osteogenic differentiation of bone marrow stromal cells(BMSCs) in vitro. Methods: Tissue adherent culture was used to isolate the BMSCs from female mandibular，and CCK-8 method was used to detect the effect of different concentrations of HSYA on the growth of BMSCs. The stemness transcription factors (NANOG，SOX2，OCT4) were detected by Western blot and RT-PCR in BMSCs supplemented with HYSA. The colony-forming efficiency was compared between HYSA group and control group. The different osteogenic ability of the HYSA group, positive group and the control group was analyzed by real-time RT-PCR，Western blot and Alizarin red staining after osteogenic induction. Results: Compared with the control group, the levels of NANOG, SOX2, and OCT4 of HYSA group were higher than that of the control group, and there was statistically significant difference. Seven days later osteogenic induction in vitro the of osteogenic differentiation genes were increased in the experimental group BMSCs. 14 days later osteogenic induction, the calcium nodules of HYSA group were more than that of the control and positive group. Conclusions: HYSA can promote the proliferative capacity of BMSCs, and induce their osteogenic differentiation, due to the participation of Runx2，OCN，OSX，OPN.

Objectives：To investigate the effect of platelet rich fibrin (PRF) membrane on periodontal healing of delayed replanted avulsed teeth. Methods：Twenty-five six-week-old male Wistar rats were used for split mouth design. After bilateral maxillary first molars extracted, dried for 30 min, self-made PRF membrane was placed in one side of the extraction socket and the avulsed teeth replanted as PRF membrane group, without PRF membrane at contra-lateral, avulsed teeth replanted as control group. On 1, 3, 7, 14 and 28 days after tooth replantation, the rats were sacrificed. Paraffin specimens of maxillary bone were prepared, sliced. HE staining was used to observe the periodontal healing of the replanted teeth and the results were statistically analyzed. Results：All the replanted teeth did not fall off. One d after tooth replantation, inflammatory infiltrate could be found in both groups, the incidence rate of periodontal inflammatory change was significantly higher in PRF membrane group than that of control group (P<0.01); After 3 days, the incidence rate of periodontal inflammatory change was still higher in PRF membrane group (P<0.01); After 7 days, the incidence rate of periodontal membrane healing was consistent in both groups(P>0.05), incidence rate of surface resorption was significantly lower in PRF membrane group than in control group (P<0.05), inflammatory resorption was consistent in both groups(P>0.05),14 days after operation, incidence rate of surface resorption was significantly higher in PRF membrane group than that of control group (P<0.05) while inflammatory resorption was significantly lower in PRF membrane group (P<0.01); 28 days after operation, periodontal healing pattern in the two groups were similar. Conclusions：In delayed avulsion teeth replantation， the application of PRF membrane did not affect the reposition and healing of replanted teeth, and could control the occurrence and development of root resorption at the initial stage. However, the long-term effect is not stable, and needs further study.

Oral cancer is the most common malignant tumor in oral and maxillofacial region in China. In recent years, although great progress has been made in the treatment of oral cancer, but its five-year survival rate is still hovering around 60%. However, early patients’ data can be as high as 90%, so early detection of oral precancerous lesions and oral cancer is of great significance to improve the survival and prognosis of patients. As a simple, convenient and non-invasive method, exfoliative cytology is increasingly used in early screening for cancer. The detection of tumor markers is of great significance to the early screening of oral cancer. With the appearance and development of liquid-based?thin-preparation technique, further progress has been made in the study of tumor markers. This paper summarized the progress in research on screening of oral cancer by detecting tumor markers in oral exfoliated cells.