BGL2和XOG1与白念珠菌生物被膜耐药性的研究

›› 2012, Vol. 3 ›› Issue (2): 74-77.

BGL2和XOG1与白念珠菌生物被膜耐药性的研究

马鸣¹,谭军艳¹,陈晓君¹,张琰²,虞丽华¹,魏昕¹

1. 江苏省口腔医院
2. 盐城市口腔医院

收稿日期:2012-04-12 修回日期:2012-05-18 出版日期:2012-06-25 发布日期:2012-06-26
通讯作者: 魏昕 E-mail:weixinart@163.com
基金资助:
省自然科学基金资助项目

The effect of BGL2 and XOG1 to Candida albicans biofilm drug resistance

Received:2012-04-12 Revised:2012-05-18 Online:2012-06-25 Published:2012-06-26

摘要/Abstract

摘要： 目的：研究白念珠菌生物被膜耐药性与BGL2和XOG1基因表达之间的关联。方法：利用浓度梯度递增法诱导构建白念珠菌氟康唑耐药株，采用KONT真菌显色MIC药敏系统鉴定耐药株模型。构建耐药株和对照株生物被膜，通过逆转录-聚合酶链反应（RT-PCR）和定量逆转录-聚合酶链反应（qRT-PCR）检测白念珠菌耐药株和对照株生物被膜细胞中葡聚糖相关基因BGL2和XOG1，并比较耐药株和对照株中BGL2和XOG1表达的差异。结果：KONT真菌显色MIC药敏系统证实耐药株最低抑菌浓度（minimal inhibitory concentration，MIC）≥ 128 μg/ml。与对照株相比，耐药株XOG1的表达明显降低（P <0.05），BGL2的表达则没有明显差异。结论：葡聚糖相关基因XOG1与白念珠菌生物被膜耐药性相关。

Abstract: Objective：To investigate the relationship between the gene BGL2, XOG1 and Candida albicans biofilm drug resistance. Methods：Candida albicans drug resistance was induced by Fluconazole with gradually increased concentration，and identified by the KONT MIC determination system. RT-PCR and qRT-PCR were used to analyze the expressions of glucan-related genes in Candida albicans SC5314 and the drug resistant strain. Results：The KONT MIC determination system showed the minimal inhibitory concentration (MIC) of the drug resistant strain was more than 128 μg/ml. Compared with the control strain, the expression of XOG1 in the drug resistant strain was significantly reduced（P <0.05）, but the expression of BGL2 was not significantly changed. Conclusions：Glucan-related gene XOG1 was related to the resistance of Candida albicans biofilm.

马鸣谭军艳陈晓君张琰虞丽华魏昕. BGL2和XOG1与白念珠菌生物被膜耐药性的研究[J]. , 2012, 3(2): 74-77.

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