Abstract: Objective：To assess the anti-microbial effect of Polident denture cleaning tablets. Methods：International standard strains were selected and cultured by using in vitro methods with quantitative suspension test. Results：That denture-cleaning tablets (Polident) solution at 40 ℃, 5 minutes on Streptococcus sanguis, Actinomyces viscosus, Klebsiella pneumoniae, Fusobacterium nucleatum, Veillonella atypica, Candida albicans, Streptococcus mutans, Porphyromonas gingivalis, Prevotella intermedia, Pseudomonas aeruginosa, Streptococcus pneumoniae had 99.9% of bacteria sterilization. Conclusions：Polident denture cleaning tablets for some oral bacteria have permanent antimicrobial effect.