17β-雌二醇对人根尖牙乳头干细胞RANKL、OPG表达影响的研究

›› 2012, Vol. 3 ›› Issue (4): 185-188.

17β-雌二醇对人根尖牙乳头干细胞RANKL、OPG表达影响的研究

王燕萍¹,曹灵²,吴锦涛¹,闫明¹,景双林¹,张光东¹,于金华¹

1. 南京医科大学口腔医学研究所
2. 本院

收稿日期:2012-10-24 修回日期:2012-11-21 出版日期:2012-12-25 发布日期:2013-01-10
通讯作者: 于金华 E-mail:yuziyi_yjh@sohu.com
基金资助:
2011江苏省卫生厅医学重点人才项目

Effect of 17β-estrodial on the expression of RANKL and OPG in human stem cells from apical papillae

Received:2012-10-24 Revised:2012-11-21 Online:2012-12-25 Published:2013-01-10

摘要/Abstract

摘要： [摘要] 目的：探讨17β-雌二醇对人根尖牙乳头干细胞（stem cells from apical papilla，SCAPs）核因子-κB受体活化因子配体（receptor activator for nuclear factor-κB ligand，RANKL）、骨保护素（osteoprotegerin，OPG）表达的影响。方法：通过酶消化法分离培养SCAPs，将SCAPs分别于对照组（普通培养基＋10 μmol/L 无水乙醇）和含0.1 μmol/L 17β-雌二醇的培养基培养3 d和7 d后，实时荧光定量PCR法和蛋白质免疫印迹法分别检测RANKL、OPG mRNA和蛋白表达。结果：17β-雌二醇刺激3 d和7 d组，OPG mRNA和蛋白表达均较对照组升高（P < 0.01），RANKL mRNA和蛋白表达均较对照组降低（P < 0.01）。结论：17β-雌二醇可能通过调节RANKL/OPG系统，参与调节SCAPs成牙/骨及破牙/骨活性。

Abstract: [Abstract] Objective：To evaluate the effects of 17β-estrodial on the expression of receptor activator of NF-κB ligand (RANKL) and osteoprotegerin (OPG) in human stem cells from apical papillae (SCAPs). Methods：SCAPs cultured in L-MEM containing 10 μmol/L alcohol, L-MEM containing 0.1 μmol/L 17β-estrodial for 3 days and 7 days were respectively collected to evaluate the expression of RANKL and OPG at the gene or protein level by real-time RT-PCR and Western blotting. Results：17β-estrodial can enhance the expression of OPG and downregulate the expression of RANKL. Conclusions：17β-estrodial may regulate the odonto/osteogenic and odonto/osteoclastic potential through RANKL/OPG system.

王燕萍曹灵吴锦涛闫明景双林张光东于金华. 17β-雌二醇对人根尖牙乳头干细胞RANKL、OPG表达影响的研究[J]. , 2012, 3(4): 185-188.

参考文献

[1]Sonoyama W, Liu Y, Yamaza T, et al.Characterization of the apical papilla and its residing stem cells from human immature permanent teeth: a pilot study[J].J Endod,2008,34(2):166-171.
[2]Ghoul-Mazgar S.RANK, RANKL and OPG expressions in a permanent molar with a replacement resorption[J].Odontology,2012,:-.
[3]Kook SH, Jang YS, Lee JC.Human periodontal ligament fibroblasts stimulate osteoclastogenesis in response to compression force through TNF-α-mediated activation of CD4+ T cells[J].J Cell Biochem.,2011,112(10):2891-2901.
[4]Booij-Vrieling HE, Tryfonidou MA, Riemers FM, et al.Inflammatory cytokines and the nuclear vitamin D receptor are implicated in the pathophysiology of dental resorptive lesions in cats[J].Vet Immunol Immunopathol,2009,132(2-4):160-166.
[5]Yamaguchi M, Aihara N, Kojima T, et al.RANKL increase in compressed periodontal ligament cells from root resorption[J].J Dent Res,2006,85(8):751-756.
[6]Wattanaroonwong N, Schoenmaker T, de Vries TJ, et al.Oestrogen inhibits osteoclast formation induced by periodontal ligament fibroblasts[J].Arch Oral Biol,2011,56(3):212-219.
[7]J?rgensen L, Vik A, Emaus N,et al.Bone loss in relation to serum levels of osteoprotegerin and nuclear factor-kappaB ligand: the Troms? Study[J].Osteoporos Int,2010,21(6):931-938.
[8]Wang S, Mu J, Fan Z, et al.Insulin-like growth factor 1 can promote the osteogenic differentiation and osteogenesis of stem cells from apical papilla[J].Stem Cell Res,2012,8(3):346-356.
[9]闫明, 曹灵, 俞艳, 等.肝细胞生长因子对根尖牙乳头干细胞增殖和矿化能力的影响[J].口腔生物医学,2011,2 (3):136-139.
[10]毕迎春, 林珠, 金作林.TNF-α对体外培养人牙囊细胞表达RANKL、OPG的影响[J].解放军医学杂志,2008,33(6):673-675.
[11]曾天舒,潘世秀,陈璐璐,等.雌激素调节大鼠去卵巢后骨髓细胞OPG、RANKL、RANK基因表达[J].中国骨质疏松杂志,2006,12(2):138-141.