SEMA3A及其受体NRP-1在SCC25/大鼠DRG共培养中对轴突芽生的影响

›› 2012, Vol. 3 ›› Issue (4): 189-192.

SEMA3A及其受体NRP-1在SCC25/大鼠DRG共培养中对轴突芽生的影响

唐子春¹,王子露²,宋晓萌²,朱江²,吴煜农²

1. 安徽省铜陵市人民医院口腔科
2. 南京医科大学附属口腔医院

收稿日期:2012-10-16 修回日期:2012-12-04 出版日期:2012-12-25 发布日期:2013-01-10
通讯作者: 吴煜农 E-mail:yunongwu@yahoo.com
基金资助:
江苏省自然科学基金;江苏省高校优势学科建设工程资助项目

The role SEMA3A and its receptor NRP-1 play in mediating the sprout of axons in co-cultures of SCC25 and rat’s DRG

Received:2012-10-16 Revised:2012-12-04 Online:2012-12-25 Published:2013-01-10

摘要/Abstract

摘要： [摘要]目的：构建人舌鳞状细胞癌/大鼠背根神经节（dorsal root ganglion，DRG）体外共培养的实验模型，研究信号素（semaphorin3A，SEMA3A）及其受体神经纤毛素1（Neuropilin-1，NRP-1）在肿瘤排斥神经的现象中可能发挥的作用。方法：鉴定SEMA3A在人舌鳞状细胞癌细胞系SCC25（squamous cell carcinoma 25）中的表达以及NRP-1在大鼠DRG神经元中的表达，建立人舌鳞状细胞癌/大鼠DRG体外共培养的实验模型，针对NRP-1靶点进行特异性拮抗，观察SCC25排斥DRG轴突生长的程度变化。结果：免疫荧光结果显示SEMA3A在SCC25存在表达而NRP-1在大鼠DRG神经元中也呈阳性表达，共培养实验结果表明DRG组织块中NRP-1被特异性拮抗后SCC25对DRG轴突的排斥程度较对照组明显减弱。结论：SEMA3A及其受体NRP-1在SCC25排斥大鼠DRG轴突生长过程中起着重要作用。

Abstract: [Abstract] Objective：To investigate the role semaphorin3A(SEMA3A) and its receptor neuropilin-1(NRP-1) play in the intriguing process that tumor grow without nervous tissue, we establish the model of cocutures of squamous cell carcinoma 25 (SCC25) and rat’s dorsal root ganglion(DRG). Methods: We tested the expression of SEMA3A and NRP-1 respectively in SCC25 and rat’s DRG. And then a model of cocutures of SCC25 and rat’s DRG was established. In the vitro cocultures, the difference of the DRG’s sprouting was observed when the medium treat with or without the antagonist of NRP-1. Results: Both the expression of SEMA3A in SCC25 and the expression of NRP-1 in rat’s DRG were positively detected, and the coculture showed that the rat’s DRG treating with the antagonist of NRP-1 sprouts better. Conclusions: SEMA3A and its receptor NRP-1 may play an important role in the process that tumor grow without nervous tissue.

中图分类号:

Q786

唐子春王子露宋晓萌朱江吴煜农. SEMA3A及其受体NRP-1在SCC25/大鼠DRG共培养中对轴突芽生的影响[J]. , 2012, 3(4): 189-192.

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