白介素10对伴放线放线杆菌内毒素诱导兔肺巨噬细胞凋亡的影响

›› 2013, Vol. 4 ›› Issue (1): 4-7.

白介素10对伴放线放线杆菌内毒素诱导兔肺巨噬细胞凋亡的影响

汪志君¹,姚军¹,罗岚¹,黄敏¹,陈帅¹,李艳芬¹,李厚轩¹,闫福华²

1. 福建医科大学口腔医学院
2. 南京大学口腔医学院

收稿日期:2012-10-23 修回日期:2013-03-08 出版日期:2013-03-25 发布日期:2013-03-30
通讯作者: 闫福华 E-mail:fhyan2005@126.com
基金资助:
国家自然科学基金

The effect of Aa-LPS and IL-10 on apoptosis of rabbit alveolar macrophages

Received:2012-10-23 Revised:2013-03-08 Online:2013-03-25 Published:2013-03-30

摘要/Abstract

摘要： 目的：为了探讨伴放线放线杆菌内毒素(Aa-LPS)体外对肺巨噬细胞的凋亡作用及加入白介素10(IL-10)后的影响。方法：经气管肺泡灌洗获得肺泡巨噬细胞，所得细胞按1×106cell/well/ml的密度随机分为空白对照组、Aa-LPS组、Aa-LPS+IL-10组。按实验分组加入Aa-LPS（1μg/ml）、IL-10（0.1μg/ml），24小时后裂解细胞，荧光定量PCR法检测促凋亡基因bax、p53和caspase-3的表达。结果：Aa-LPS组凋亡基因bax、caspase-3的表达较空白对照组明显升高（P<0.05），p53的表达与空白对照组比较，无统计学意义（P>0.05）。Aa-LPS+IL-10组的凋亡基因bax、p53、caspase-3的表达较Aa-LPS组降低（P<0.05）。结论：Aa-LPS体外对肺巨噬细胞有促凋亡作用，IL-10可抑制Aa-LPS的促凋亡作用，其机制可能与细胞凋亡的线粒体途径有关。

Abstract: Objective: To investigate the effect of Aa-LPS and IL-10 on apoptosis of Alveolar macrophages in vitro .Methods:Alveolar macrophages(AM) were harvested from the bronchoalveolar lavage fluid(BALF).These cells were randomly divided into three groups: control group, Aa-LPS group and Aa-LPS +IL-10 group. The number of cells was 1×106/ml in each group. Then these cells were given Aa-LPS(1μg/ml),IL-10（0.1μg/ml）.After 24 hours,the cells were harvested to extract RNA. Finally, RT-PCR was used to detect the expression of gene bax,p53 and caspase-3.Results:The results showed that the expression of bax and caspase-3 in the Aa-LPS group were significantly higher than the control group（P<0.05）.The expression of p53 was not statistical significant compared with the control group（P>0.05）. IL-10 treatment significantly inhibited macrophagocyte apoptosis and suppressed the expression of bax,p53 and caspase-3（P<0.05）.Conclusions: Aa-LPS can promote alveolar macrophages apoptosis in vitro and IL-10 can suppress the effect of Aa-LPS. The mechanism seemed related to the mitochondria-dependent pathway.

中图分类号:

R392.12

汪志君姚军罗岚黄敏陈帅李艳芬李厚轩闫福华. 白介素10对伴放线放线杆菌内毒素诱导兔肺巨噬细胞凋亡的影响[J]. , 2013, 4(1): 4-7.

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