卵巢去势对大鼠牙髓组织成牙/成骨能力的影响

›› 2013, Vol. 4 ›› Issue (3): 117-120.

卵巢去势对大鼠牙髓组织成牙/成骨能力的影响

王燕萍¹,宋卫健²,曹灵³,于金华¹

1. 南京医科大学口腔医学研究所
2. 苏州口腔医院
3. 南京医科大学附属口腔医院

收稿日期:2013-08-06 修回日期:2013-08-23 出版日期:2013-09-25 发布日期:2013-10-22
通讯作者: 于金华 E-mail:yuziyi_yjh@sohu.com
基金资助:
2011江苏省卫生厅医学重点人才项目;2011江苏高校优势学科建设工程资助项目

Effects of ovariectomy on the odonto/osteogenic capacity of rat incisor pulps

Received:2013-08-06 Revised:2013-08-23 Online:2013-09-25 Published:2013-10-22

摘要/Abstract

摘要： 目的：观察卵巢去势一个月对大鼠切牙牙髓组织中成牙、成骨指标的影响。方法：将10只成年雌性SD大鼠（8周龄）随机分为假手术组及卵巢去势组，每组各5只；在全麻下，将去势组大鼠双侧卵巢摘除，假手术组行相同切口，保留卵巢；一个月后，处死大鼠，分离牙髓组织，通过实时定量RT-PCR及Western blot方法检测两组牙髓组织中相关成牙、成骨指标的表达。结果：去势组牙髓组织中，Runt相关转录因子2（Runt-related transcription factor 2，RUNX2）、骨细胞特异性转录因子（Osterix，OSX）、骨钙素（Osteocalcin，OCN）、牙本质涎蛋白（dentin sialoprotein，DSP）、牙本质涎磷蛋白（dentin sialophosphoprotein，DSPP）的基因和/或蛋白表达等成牙及成骨指标均较假手术组牙髓低,而碱性磷酸酶（alkaline phosphatase，ALP）的表达无明显改变。结论：卵巢去势一个月可以明显降低大鼠切牙牙髓组织中成牙、成骨指标的表达，提示卵巢去势下调了牙髓细胞的成牙及成骨能力。

Abstract: Objective：To investigate the effects of ovariectomy on the expression of odonto/osteogenic markers in rat incisor pulps. Methods：10 8-week-old female SD rats were randomly divided into 2 groups: Ovariectomized group (OVX, n=5) and Sham-operation group (n=5). All rats were anesthetized and both ovaries of OVX-rats were gently removed. Sham-operation rats were treated with the same incisions to expose the ovaries but without any hurt to them. One month after the operation, the incisor pulps were respectively isolated. Real-time RT-PCR and western blot were performed to evaluate the expression of odonto/osteogenic markers in the two groups. Results：The expression of odonto/osteogenic markers (i.e., RUNX2/RUNX2, OSX/OSX, OCN/OCN, and DSPP/DSP) in OVX group was significantly weaker than that in Sham group. However，the expression of ALP was not significantly changed. Conclusions：Ovariectomy can downregulate the expression of odonto/osteogenic markers in dental pulps, indicating that ovariectomy decreased the odonto/osteogenic capacity of dental pulp cells.

中图分类号:

R780.2

王燕萍宋卫健曹灵于金华. 卵巢去势对大鼠牙髓组织成牙/成骨能力的影响[J]. , 2013, 4(3): 117-120.

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